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Science 12 February 1988:
Vol. 239. no. 4841, pp. 783 - 785
DOI: 10.1126/science.3422517
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Articles

Science, Vol 239, Issue 4841, 783-785
Copyright © 1988 by American Association for the Advancement of Science

articles

Mesoderm induction in amphibians: the role of TGF-beta 2-like factors

F Rosa, AB Roberts, D Danielpour, LL Dart, MB Sporn, and IB Dawid

Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892.

Mesoderm induction in the amphibian embryo can be studied by exposing animal region explants (destined to become ectoderm) to appropriate stimuli and assaying the appearance of mesodermal products like alpha-actin messenger RNA. Transforming growth factor beta 2 (TGF-beta 2), but not TGF-beta 1, was active in alpha-actin induction, while addition of fibroblast growth factor had a small synergistic effect. Medium conditioned by Xenopus XTC cells (XTC-CM), known to have powerful mesoderm-inducing activity, was shown to contain TGF-beta-like activity as measured by a radioreceptor binding assay, colony formation in NRK cells, and growth inhibition in CCL64 cells. The activity of XTC-CM in mesoderm induction and in growth inhibition of CCL64 cells was inhibited partially by antibodies to TGF-beta 2 but not by antibodies to TGF-beta 1. Thus, a TGF-beta 2-like molecule may be involved in mesoderm induction.


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