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Science 12 February 1988:
Vol. 239. no. 4841, pp. 778 - 780
DOI: 10.1126/science.2448878
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Articles

Science, Vol 239, Issue 4841, 778-780
Copyright © 1988 by American Association for the Advancement of Science

articles

Effects of intracellular free magnesium on calcium current in isolated cardiac myocytes

RE White and HC Hartzell

Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322.

Magnesium ions play a fundamental role in cellular function, but the effects of changes in the concentration of intracellular ionized magnesium ([Mg2+]i) on cell physiology have only recently received experimental attention. Increasing [Mg2+]i from 0.3 to 3.0 mM in cardiac cells by internal perfusion has only small effects on the basal voltage-gated calcium current (ICa) or on ICa elevated by dihydropyridine calcium channel agonists. In contrast, ICa elevated by cyclic adenosine monophosphate (cAMP)-dependent phosphorylation decreases by more than 50 percent. The effect of [Mg2+]i is not due to changes in the concentration of cAMP or in the velocity of phosphorylation but rather appears to be a direct effect on the phosphorylated channel or on channel dephosphorylation.


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