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Science 12 February 1988:
Vol. 239. no. 4841, pp. 753 - 759
DOI: 10.1126/science.3340856
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Articles

Science, Vol 239, Issue 4841, 753-759
Copyright © 1988 by American Association for the Advancement of Science

articles

Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane

MA Ferguson, SW Homans, RA Dwek, and TW Rademacher

Oxford Oligosaccharide Group, Department of Biochemistry, University of Oxford, England.

Two forms of protein-membrane anchor have been described for the externally disposed glycoproteins of eukaryotic plasma membranes; namely, the hydrophobic transmembrane polypeptide and the complex glycosylphosphatidylinositol (G-PI) moiety. The chemical structures of the major species of G-PI anchors found on a single variant surface glycoprotein (VSG) of the parasitic protozoan Trypanosoma brucei were determined by a combination of nuclear magnetic resonance spectroscopy, mass spectrometry, chemical modification, and exoglycosidase digestions. The G-PI anchor was found to be heterogeneous with respect to monosaccharide sequence, and several novel glycosidic linkages were present. The results are pertinent to the mechanism of the biosynthesis of G-PI anchors.


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Purification and Structural Characterization of a Filamentous, Mucin-like Proteophosphoglycan Secreted by Leishmania Parasites.
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T. K. Smith, S. Cottaz, John. S. Brimacombe, and M. A. J. Ferguson (1996)
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Structures of the Glycosyl-phosphatidylinositol Anchors of Porcine and Human Renal Membrane Dipeptidase.
I. A. Brewis, M. A. J. Ferguson, A. Mehlert, A. J. Turner, and N. M. Hooper (1995)
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Structural Analysis of the Mannan Region of Lipoarabinomannan from Mycobacterium bovis BCG.
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Primary Structure of CD52.
A. Treumann, M. R. Lifely, P. Schneider, and M. A. J. Ferguson (1995)
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Glycan Requirements of Glycosylphosphatidylinositol Phospholipase C from Trypanosoma brucei.
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J. Biol. Chem. 270, 2517-2524
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The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.
T Miyata, J Takeda, Y Iida, N Yamada, N Inoue, M Takahashi, K Maeda, T Kitani, and T Kinoshita (1993)
Science 259, 1318-1320
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Carboxyl terminus structural requirements for glycosyl-phosphatidylinositol anchor addition to cell surface proteins.
C Beghdadi-Rais, M Schreyer, M Rousseaux, P Borel, R. Eisenberg, G. Cohen, C Bron, and N Fasel (1993)
J. Cell Sci. 105, 831-840
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Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene.
R DeGasperi, L. Thomas, E Sugiyama, H. Chang, P. Beck, P Orlean, C Albright, G Waneck, J. Sambrook, C. Warren, et al. (1990)
Science 250, 988-991
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Structural Conservation of Neurotropism-associated VspA within the Variable Borrelia Vsp-OspC Lipoprotein Family.
W. R. Zuckert, T. A. Kerentseva, C. L. Lawson, and A. G. Barbour (2001)
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