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Science 5 February 1988:
Vol. 239. no. 4840, pp. 640 - 643
DOI: 10.1126/science.2829356
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Articles

Science, Vol 239, Issue 4840, 640-643
Copyright © 1988 by American Association for the Advancement of Science

articles

Mitogenesis in response to PDGF and bombesin abolished by microinjection of antibody to PIP2

K Matuoka, K Fukami, O Nakanishi, S Kawai, and T Takenawa

Department of Pharmacology, Tokyo Metropolitan Institute of Gerontology, Japan.

The turnover of phosphatidylinositol 4,5-bisphosphate (PIP2) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including mitogens, but decisive evidence for the idea has not been obtained. In the present study, a monoclonal antibody to PIP2 was microinjected into the cytoplasm of NIH 3T3 cells before or after exposure to mitogens. The antibody completely abolished nuclear labeling with [3H]thymidine induced by platelet-derived growth factor and bombesin, but not by fibroblast growth factor, epidermal growth factor, insulin, or serum. The findings strongly suggest that PIP2 breakdown is crucial in the elicitation and sustaining of cell proliferation induced by some types of mitogens such as platelet-derived growth factor and bombesin.


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