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Science 5 February 1988:
Vol. 239. no. 4840, pp. 637 - 640
DOI: 10.1126/science.3257585
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Articles

Science, Vol 239, Issue 4840, 637-640
Copyright © 1988 by American Association for the Advancement of Science

articles

Cells process exogenous proteins for recognition by cytotoxic T lymphocytes

JW Yewdell, Bennink JR, and Y Hosaka

Wistar Institute for Anatomy and Biology, Philadelphia, PA 19104.

Cells exposed to intact, noninfectious influenza virus were shown to be recognized by class I-restricted anti-influenza cytotoxic T lymphocytes (CTLs). Both internal and external proteins derived from virions were processed by cells for CTL recognition. Sensitization required the inactivation of viral neuraminidase activity and could be inhibited by preventing fusion of viral and cellular membranes. These findings are important in designing vaccines to elicit CTL responses, since they demonstrate that cells can process intact, exogenous proteins for recognition by CTLs and suggest that such processing depends on introduction of exogenous proteins into the cytoplasm.


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