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Science 22 January 1988:
Vol. 239. no. 4838, pp. 387 - 390
DOI: 10.1126/science.239.4838.387
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Articles

Enzymatic Oxidation of Cholesterol Aggregates in Supercritical Carbon Dioxide

T. W. RANDOLPH 1, D. S. CLARK 1, H. W. BLANCH 1, and J. M. PRAUSNITZ 1

1 Department of Chemical Engineering, University of California, Berkeley, CA 94720.

Fundamental studies of enzyme-solvent interactions can be conducted with supercritical fluids because small changes in pressure or temperature may bring about great changes in the properties of a single solvent near its critical point. Cholesterol oxidase is active in supercritical carbon dioxide and supercritical carbon dioxide-cosolvent mixtures. Variations in solvent power caused by pressure changes or by the addition of dopants affected the rate of enzymatic oxidation of cholesterol by altering the structure of cholesterol aggregates.

Submitted on August 28, 1987
Accepted on December 7, 1987


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Enzymatic catalysts in organic synthesis.
C. Wong (1989)
Science 244, 1145-1152
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