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Science 15 January 1988:
Vol. 239. no. 4837, pp. 293 - 295
DOI: 10.1126/science.3276005
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Articles

Science, Vol 239, Issue 4837, 293-295
Copyright © 1988 by American Association for the Advancement of Science

articles

A 13-kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin

RE Dewey, JN Siedow, DH Timothy, and CS Levings 3rd

Department of Crop Science, North Carolina State University, Raleigh 27695.

The Texas male-sterile cytoplasm (cms-T) of maize carries the cytoplasmically inherited trait of male sterility. Mitochondria isolated from cms-T maize are specifically sensitive to a toxin (BmT-toxin) produced by the fungal pathogen Bipolaris maydis, race T, and the carbamate insecticide methomyl. A mitochondrial gene unique to cms-T maize, which produces a 13-kilodalton polypeptide associated with cytoplasmic male sterility, was expressed in Escherichia coli. After addition of BmT-toxin or methomyl, inhibition of whole cell respiration and swelling of spheroplasts were observed in Escherichia coli cultures producing the novel mitochondrial protein; these effects are similar to those observed with isolated cms-T mitochondria. The amino-terminal region of the 13-kilodalton polypeptide appears to be essential for proper interaction with the BmT-toxin and methomyl. These results implicate the 13-kilodalton polypeptide in conferring toxin sensitivity to mitochondria of cms-T maize.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cytoplasmic Male Sterility of Rice with Boro II Cytoplasm Is Caused by a Cytotoxic Peptide and Is Restored by Two Related PPR Motif Genes via Distinct Modes of mRNA Silencing.
Z. Wang, Y. Zou, X. Li, Q. Zhang, L. Chen, H. Wu, D. Su, Y. Chen, J. Guo, D. Luo, et al. (2006)
PLANT CELL 18, 676-687
   Abstract »    Full Text »    PDF »
Rearrangements of Human Mitochondrial DNA (mtDNA): New Insights into the Regulation of mtDNA Copy Number and Gene Expression.
Y. Tang, E. A. Schon, E. Wilichowski, M. E. Vazquez-Memije, E. Davidson, and M. P. King (2000)
Mol. Biol. Cell 11, 1471-1485
   Abstract »    Full Text »
The Texas Cytoplasm of Maize: Cytoplasmic Male Sterility and Disease Susceptibility.
C. S. Levings III (1990)
Science 250, 942-947
   Abstract »    PDF »
Sensitivity to Alternaria alternata toxin in citrus because of altered mitochondrial RNA processing.
K. Ohtani, H. Yamamoto, and K. Akimitsu (2002)
PNAS 99, 2439-2444
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)