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Science 15 January 1988:
Vol. 239. no. 4837, pp. 276 - 278
DOI: 10.1126/science.2447650
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Articles

Science, Vol 239, Issue 4837, 276-278
Copyright © 1988 by American Association for the Advancement of Science

articles

Bacterial motility: membrane topology of the Escherichia coli MotB protein

SY Chun and JS Parkinson

Biology Department, University of Utah, Salt Lake City 84112.

The MotB protein of Escherichia coli is an essential component of the force generators that couple proton movement across the cytoplasmic membrane to rotation of the flagellar motors. The membrane topology of MotB was examined to explore the possibility that it might form a proton channel. MotB--alkaline phosphatase fusion proteins were constructed to identify likely periplasmic domains of the MotB molecule. Fusions distal to a putative membrane-spanning segment near the amino terminus of MotB exhibited alkaline phosphatase activity, indicating that an extensive carboxyl-terminal portion of MotB may be located on the periplasmic side of the membrane. Protease treatment of MotB in spheroplasts confirmed this view. The simple transmembrane organization of MotB is difficult to reconcile with a role as a proton conductor.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Clusters of Charged Residues at the C Terminus of MotA and N Terminus of MotB Are Important for Function of the Escherichia coli Flagellar Motor.
E. R. Hosking and M. D. Manson (2008)
J. Bacteriol. 190, 5517-5521
   Abstract »    Full Text »    PDF »
Crystal structure of the cell wall anchor domain of MotB, a stator component of the bacterial flagellar motor: Implications for peptidoglycan recognition.
A. Roujeinikova (2008)
PNAS 105, 10348-10353
   Abstract »    Full Text »    PDF »
Roles of Charged Residues in the C-Terminal Region of PomA, a Stator Component of the Na+-Driven Flagellar Motor.
M. Obara, T. Yakushi, S. Kojima, and M. Homma (2008)
J. Bacteriol. 190, 3565-3571
   Abstract »    Full Text »    PDF »
Characterization of the Periplasmic Domain of MotB and Implications for Its Role in the Stator Assembly of the Bacterial Flagellar Motor.
S. Kojima, Y. Furukawa, H. Matsunami, T. Minamino, and K. Namba (2008)
J. Bacteriol. 190, 3314-3322
   Abstract »    Full Text »    PDF »
Mutational Analysis of the Flagellar Protein FliG: Sites of Interaction with FliM and Implications for Organization of the Switch Complex.
P. N. Brown, M. Terrazas, K. Paul, and D. F. Blair (2007)
J. Bacteriol. 189, 305-312
   Abstract »    Full Text »    PDF »
The Three-Dimensional Structure of the Flagellar Rotor from a Clockwise-Locked Mutant of Salmonella enterica Serovar Typhimurium..
D. R. Thomas, N. R. Francis, C. Xu, and D. J. DeRosier (2006)
J. Bacteriol. 188, 7039-7048
   Abstract »    Full Text »    PDF »
Organization of FliN Subunits in the Flagellar Motor of Escherichia coli..
K. Paul and D. F. Blair (2006)
J. Bacteriol. 188, 2502-2511
   Abstract »    Full Text »    PDF »
The Ability of Proteus mirabilis To Sense Surfaces and Regulate Virulence Gene Expression Involves FliL, a Flagellar Basal Body Protein.
R. Belas and R. Suvanasuthi (2005)
J. Bacteriol. 187, 6789-6803
   Abstract »    Full Text »    PDF »
FliG Subunit Arrangement in the Flagellar Rotor Probed by Targeted Cross-Linking.
B. J. Lowder, M. D. Duyvesteyn, and D. F. Blair (2005)
J. Bacteriol. 187, 5640-5647
   Abstract »    Full Text »    PDF »
Interactions of MotX with MotY and with the PomA/PomB Sodium Ion Channel Complex of the Vibrio alginolyticus Polar Flagellum.
M. Okabe, T. Yakushi, and M. Homma (2005)
J. Biol. Chem. 280, 25659-25664
   Abstract »    Full Text »    PDF »
Evidence for Two Flagellar Stators and Their Role in the Motility of Pseudomonas aeruginosa.
C. M. Toutain, M. E. Zegans, and G. A. O'Toole (2005)
J. Bacteriol. 187, 771-777
   Abstract »    Full Text »    PDF »
Deletion Analysis of the Carboxyl-Terminal Region of the PomB Component of the Vibrio alginolyticus Polar Flagellar Motor.
T. Yakushi, N. Hattori, and M. Homma (2005)
J. Bacteriol. 187, 778-784
   Abstract »    Full Text »    PDF »
Concerted Effects of Amino Acid Substitutions in Conserved Charged Residues and Other Residues in the Cytoplasmic Domain of PomA, a Stator Component of Na+-Driven Flagella.
H. Fukuoka, T. Yakushi, and M. Homma (2004)
J. Bacteriol. 186, 6749-6758
   Abstract »    Full Text »    PDF »
The Complex Flagellar Torque Generator of Pseudomonas aeruginosa.
T. B. Doyle, A. C. Hawkins, and L. L. McCarter (2004)
J. Bacteriol. 186, 6341-6350
   Abstract »    Full Text »    PDF »
Rusty, Jammed, and Well-Oiled Hinges: Mutations Affecting the Interdomain Region of FliG, a Rotor Element of the Escherichia coli Flagellar Motor.
S. M. Van Way, S. G. Millas, A. H. Lee, and M. D. Manson (2004)
J. Bacteriol. 186, 3173-3181
   Abstract »    Full Text »    PDF »
Isolation of Vibrio alginolyticus sodium-driven flagellar motor complex composed of PomA and PomB solubilized by sucrose monocaprate.
T. Yakushi, M. Kojima, and M. Homma (2004)
Microbiology 150, 911-920
   Abstract »    Full Text »    PDF »
Downregulation of the motA gene delays the escape of the obligate predator Bdellovibrio bacteriovorus 109J from bdelloplasts of bacterial prey cells.
R. S. Flannagan, M. A. Valvano, and S. F. Koval (2004)
Microbiology 150, 649-656
   Abstract »    Full Text »    PDF »
Multimeric Structure of the PomA/PomB Channel Complex in the Na+-Driven Flagellar Motor of Vibrio alginolyticus.
T. Yorimitsu, M. Kojima, T. Yakushi, and M. Homma (2004)
J. Biochem. 135, 43-51
   Abstract »    Full Text »    PDF »
A Slow-Motility Phenotype Caused by Substitutions at Residue Asp31 in the PomA Channel Component of a Sodium-Driven Flagellar Motor.
S. Kojima, T. Shoji, Y. Asai, I. Kawagishi, and M. Homma (2000)
J. Bacteriol. 182, 3314-3318
   Abstract »    Full Text »
Functional Reconstitution of the Na+-driven Polar Flagellar Motor Component of Vibrio alginolyticus.
K. Sato and M. Homma (2000)
J. Biol. Chem. 275, 5718-5722
   Abstract »    Full Text »    PDF »
Hybrid Motor with H+- and Na+-Driven Components Can Rotate Vibrio Polar Flagella by Using Sodium Ions.
Y. Asai, I. Kawagishi, R. E. Sockett, and M. Homma (1999)
J. Bacteriol. 181, 6332-6338
   Abstract »    Full Text »
Functional Interaction between PomA and PomB, the Na+-Driven Flagellar Motor Components of Vibrio alginolyticus.
T. Yorimitsu, K. Sato, Y. Asai, I. Kawagishi, and M. Homma (1999)
J. Bacteriol. 181, 5103-5106
   Abstract »    Full Text »
Function of Proline Residues of MotA in Torque Generation by the Flagellar Motor of Escherichia coli.
T. F. Braun, S. Poulson, J. B. Gully, J. C. Empey, S. Van Way, A. Putnam, and D. F. Blair (1999)
J. Bacteriol. 181, 3542-3551
   Abstract »    Full Text »
The Mxi-Spa Type III Secretory Pathway of Shigella flexneri Requires an Outer Membrane Lipoprotein, MxiM, for Invasin Translocation.
R. Schuch and A. T. Maurelli (1999)
Infect. Immun. 67, 1982-1991
   Abstract »    Full Text »    PDF »
The Polar Flagellar Motor of Vibrio cholerae Is Driven by an Na+ Motive Force.
S. Kojima, K. Yamamoto, I. Kawagishi, and M. Homma (1999)
J. Bacteriol. 181, 1927-1930
   Abstract »    Full Text »
Electrostatic interactions between rotor and stator in the bacterial flagellar motor.
J. Zhou, S. A. Lloyd, and D. F. Blair (1998)
PNAS 95, 6436-6441
   Abstract »    Full Text »    PDF »
Function of Protonatable Residues in the Flagellar Motor of Escherichia coli: a Critical Role for Asp 32 of MotB.
J. Zhou, L. L. Sharp, H. L. Tang, S. A. Lloyd, S. Billings, T. F. Braun, and D. F. Blair (1998)
J. Bacteriol. 180, 2729-2735
   Abstract »    Full Text »
Bacterial Microprocessing.
H.C. Berg (1990)
Cold Spring Harb Symp Quant Biol 55, 539-545
   Abstract »    PDF »
Restoration of torque in defective flagellar motors.
D. Blair and H. Berg (1988)
Science 242, 1678-1681
   Abstract »    PDF »
Multimeric Structure of PomA, a Component of the Na+-driven Polar Flagellar Motor of Vibrio alginolyticus.
K. Sato and M. Homma (2000)
J. Biol. Chem. 275, 20223-20228
   Abstract »    Full Text »    PDF »


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