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Science 8 January 1988:
Vol. 239. no. 4836, pp. 184 - 187
DOI: 10.1126/science.3422113
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Articles

Science, Vol 239, Issue 4836, 184-187
Copyright © 1988 by American Association for the Advancement of Science

articles

A quantitative bioassay for HIV-1 based on trans-activation

BK Felber and GN Pavlakis

National Cancer Institute, Frederick Cancer Research Facility, Bionetics Research, Inc., MD 21701.

A bioassay that is based on trans-activation has been developed for the detection and quantitation of the human immunodeficiency virus type 1 (HIV-1). Indicator cell lines were constructed that contain the HIV-1 long terminal repeat ligated to the chloramphenicol acetyltransferase (CAT) gene. Infection of these cells by HIV activates the expression of CAT protein. Isolates of HIV-1 with divergent nucleotide sequences activated the indicator cell lines to a similar extent, approximately 500- to 1000-fold. Human T cell lymphotropic viruses types 1 and 2, equine infectious anemia virus, and herpes simplex virus 1 did not activate the indicator cell lines. Isolates of simian immunodeficiency virus and human T cell lymphotropic virus type 4 activated these cells to a much lesser extent, which suggests that these viruses contain similar, but distinct, trans-activators. This assay can be used for the detection, quantitation, and typing of HIV and for studying the effect of drugs on the replication of HIV in different cellular backgrounds.


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