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Science 1 January 1988:
Vol. 239. no. 4835, pp. 61 - 64
DOI: 10.1126/science.2962287
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Articles

Science, Vol 239, Issue 4835, 61-64
Copyright © 1988 by American Association for the Advancement of Science

articles

Molecular crowding on the cell surface

TA Ryan, J Myers, D Holowka, B Baird, and WW Webb

Department of Physics, Cornell University, Ithaca, NY 14853.

Strong steric interactions among proteins on crowded living cell surfaces were revealed by measurements of the equilibrium spatial distributions of proteins in applied potential gradients. The fraction of accessible surface occupied by mobile surface proteins can be accurately represented by including steric exclusion in the statistical thermodynamic analysis of the data. The analyses revealed enhanced, concentration-dependent activity coefficients, implying unanticipated thermodynamic activity even at typical cell surface receptor concentrations.


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