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Science 18 December 1987:
Vol. 238. no. 4834, pp. 1720 - 1722
DOI: 10.1126/science.3686010
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Articles

Science, Vol 238, Issue 4834, 1720-1722
Copyright © 1987 by American Association for the Advancement of Science

articles

Sodium-calcium exchange in heart: membrane currents and changes in [Ca2+]i

L Barcenas-Ruiz, DJ Beuckelmann, and WG Wier

Department of Physiology, University of Maryland School of Medicine, Baltimore.

Recordings have been made of changes in intracellular calcium ion concentration ([Ca2+]i) that can be attributed to the operation of an electrogenic, voltage-dependent sodium-calcium (Na-Ca) exchanger in mammalian heart cells. Guinea pig ventricular myocytes under voltage clamp were perfused internally with fura-2, a fluorescent Ca2+-indicator, and changes in [Ca2+]i and membrane current that resulted from Na-Ca exchange were identified through the use of various organic channel blockers and impermeant ions. Depolarization of cells elicited slow increases in [Ca2+]i, with the maximum increase depending on internal [Na+], external [Ca2+], and membrane voltage. Repolarization was associated with net Ca2+ efflux and a decline in the inward current that developed instantaneously upon repolarization. The relation between [Ca2+]i and current was linear, and the slope was made steeper by hyperpolarization.


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