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Science 11 December 1987:
Vol. 238. no. 4833, pp. 1578 - 1581
DOI: 10.1126/science.2825352
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Articles

Science, Vol 238, Issue 4833, 1578-1581
Copyright © 1987 by American Association for the Advancement of Science

articles

Identification of nuclear receptors for VIP on a human colonic adenocarcinoma cell line

MB Omary and MF Kagnoff

Department of Medicine, University of California, San Diego, La Jolla 92093.

Vasoactive intestinal peptide (VIP) is a neuropeptide with broad tissue distribution. Although its precise function is unknown, it is thought to exert its effect, at least in part, by interacting with cell surface receptors. Nuclear receptors for VIP have now been identified by specific binding of 125I-labeled VIP to nuclei of a human colonic adenocarcinoma cell line (HT29) and by cross-linking of 125I-labeled VIP to its receptor on intact nuclei. In contrast, 125I-labeled transferrin shows only background binding to nuclei but significant binding to intact cells. Purity of the isolated nuclei was further substantiated by electron microscopy. The apparent molecular sizes of the VIP--cross-linked nuclear and cell surface receptors are similar but not identical.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
NT Agonist Regulates Expression of Nuclear High-affinity Neurotensin Receptors.
M. Toy-Miou-Leong, C.-M. Bachelet, D. Pelaprat, W. Rostene, and P. Forgez (2004)
J. Histochem. Cytochem. 52, 335-346
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