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Science 11 December 1987:
Vol. 238. no. 4833, pp. 1563 - 1565
DOI: 10.1126/science.3685993
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Articles

Science, Vol 238, Issue 4833, 1563-1565
Copyright © 1987 by American Association for the Advancement of Science

articles

Genetic ablation: targeted expression of a toxin gene causes microphthalmia in transgenic mice

ML Breitman, S Clapoff, J Rossant, LC Tsui, LM Glode, IH Maxwell, and A Bernstein

Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.

Lineage-specific regulatory elements can be used to direct expression of a variety of genes to specific tissues in transgenic mice. If the hybrid constructs contain a gene encoding a cytotoxic gene product, then genetic ablation of a specific cell lineage can be achieved. We have generated six transgenic mice by introducing into fertilized eggs the mouse gamma 2-crystallin promoter fused to the coding region of the diphtheria toxin A-chain gene. Three of these mice and all the transgenic offspring analyzed were microphthalmic. The lenses of these mice displayed considerable heterogeneity: some were almost normal morphologically but reduced in size, whereas others were grossly aberrant and deficient in nuclear fiber cells. These studies indicate that programmed ablation of specific cell types can be stably transmitted through the germ line.


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