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Science 4 December 1987:
Vol. 238. no. 4832, pp. 1419 - 1423
DOI: 10.1126/science.2446391
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Articles

Science, Vol 238, Issue 4832, 1419-1423
Copyright © 1987 by American Association for the Advancement of Science

articles

Effect of membrane potential changes on the calcium transient in single rat cardiac muscle cells

MB Cannell, Berlin JR, and WJ Lederer

Department of Pharmacology, University of Miami School of Medicine, FL 33136.

The mechanism that links membrane potential changes to the release of calcium from internal stores to cause contraction of cardiac cells is unclear. By using the calcium indicator fura-2 under voltage-clamp conditions, changes in intracellular calcium could be monitored in single rat ventricular cells while controlling membrane potential. The voltage dependence of the depolarization-induced increase in intracellular calcium was not the same as that of the calcium current (Isi), which suggests that only a small fraction of Isi is required to trigger calcium release from the sarcoplasmic reticulum. In addition, sarcoplasmic reticulum calcium release may be partly regulated by membrane potential, since repolarization could terminate the rise in intracellular calcium. Thus, changes in the action potential will have immediate effects on the time course of the calcium transient beyond those associated with its effects on Isi.


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