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Science 4 December 1987: Vol. 238. no. 4832, pp. 1386 - 1392 DOI: 10.1126/science.2825349
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Articles
Science, Vol 238, Issue 4832, 1386-1392
Copyright © 1987 by American Association for the Advancement of Science
Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1
D Bohmann,
TJ Bos,
A Admon,
T Nishimura,
PK Vogt,
and
R Tjian
Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley, CA 94720.
Nuclear oncogene products have the potential to induce alterations in gene regulation leading to the genesis of cancer. The biochemical mechanisms by which nuclear oncoproteins act remain unknown. Recently, an oncogene, v-jun, was found to share homology with the DNA binding domain of a yeast transcription factor, GCN4. Furthermore, GCN4 and the phorbol ester-inducible enhancer binding protein, AP-1, recognize very similar DNA sequences. The human proto-oncogene c-jun has now been isolated, and the deduced amino acid sequence indicates more than 80 percent identity with v-jun. Expression of cloned c-jun in bacteria produced a protein with sequence-specific DNA binding properties identical to AP-1. Antibodies raised against two distinct peptides derived from v-jun reacted specifically with human AP-1. In addition, partial amino acid sequence of purified AP-1 revealed tryptic peptides in common with the c-jun protein. The structural and functional similarities between the c-jun product and the enhancer binding protein suggest that AP-1 may be encoded by c-jun. These findings demonstrate that the proto-oncogene product of c-jun interacts directly with specific target DNA sequences to regulate gene expression, and therefore it may now be possible to identify genes under the control of c-jun that affect cell growth and neoplasia.
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- Regulation of transcription factor AP-2 by the morphogen retinoic acid and by second messengers..
- B Luscher, P J Mitchell, T Williams, and R Tjian (1989)
Genes & Dev.
3, 1507-1517
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- Activation of transcription by v-myb: evidence for two different mechanisms..
- K H Klempnauer, H Arnold, and H Biedenkapp (1989)
Genes & Dev.
3, 1582-1589
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- Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes..
- R J Christy, V W Yang, J M Ntambi, D E Geiman, W H Landschulz, A D Friedman, Y Nakabeppu, T J Kelly, and M D Lane (1989)
Genes & Dev.
3, 1323-1335
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- Purification of a set of cellular polypeptides that bind to the purine-rich cis-regulatory element of herpes simplex virus immediate early genes..
- K L LaMarco and S L McKnight (1989)
Genes & Dev.
3, 1372-1383
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- Specific expression of nuclear proto-oncogenes before entry into meiotic prophase of spermatogenesis.
- H Wolfes, K Kogawa, C. Millette, and G. Cooper (1989)
Science
245, 740-743
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- Preferential heterodimer formation by isolated leucine zippers from fos and jun.
- E. O'Shea, R Rutkowski, W. Stafford 3rd, and P. Kim (1989)
Science
245, 646-648
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- Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer..
- S L Forsburg and L Guarente (1989)
Genes & Dev.
3, 1166-1178
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- Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
- P. Mitchell and R Tjian (1989)
Science
245, 371-378
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- Co-localization of elements required for phorbol ester stimulation and glucocorticoid repression of proliferin gene expression..
- J C Mordacq and D I Linzer (1989)
Genes & Dev.
3, 760-769
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- Fos-Jun interaction: mutational analysis of the leucine zipper domain of both proteins..
- L J Ransone, J Visvader, P Sassone-Corsi, and I M Verma (1989)
Genes & Dev.
3, 770-781
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- AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells.
- L. Bernstein and N. Colburn (1989)
Science
244, 566-569
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- RNA-binding proteins as developmental regulators..
- R J Bandziulis, M S Swanson, and G Dreyfuss (1989)
Genes & Dev.
3, 431-437
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- Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
- R Turner and R Tjian (1989)
Science
243, 1689-1694
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- Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
- R Gentz, F. Rauscher 3rd, C Abate, and T Curran (1989)
Science
243, 1695-1699
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- Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins..
- W S Moye-Rowley, K D Harshman, and C S Parker (1989)
Genes & Dev.
3, 283-292
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- A FOS protein is present in a complex that binds a negative regulator of MYC..
- N Hay, M Takimoto, and J M Bishop (1989)
Genes & Dev.
3, 293-303
| Abstract »
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- Growth factors and membrane depolarization activate distinct programs of early response gene expression: dissociation of fos and jun induction..
- D P Bartel, M Sheng, L F Lau, and M E Greenberg (1989)
Genes & Dev.
3, 304-313
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- The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein..
- W F Odenwald, J Garbern, H Arnheiter, E Tournier-Lasserve, and R A Lazzarini (1989)
Genes & Dev.
3, 158-172
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