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Science 4 December 1987:
Vol. 238. no. 4832, pp. 1386 - 1392
DOI: 10.1126/science.2825349

Articles

Science, Vol 238, Issue 4832, 1386-1392
Copyright © 1987 by American Association for the Advancement of Science


articles

Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1

D Bohmann, TJ Bos, A Admon, T Nishimura, PK Vogt, and R Tjian

Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley, CA 94720.

Nuclear oncogene products have the potential to induce alterations in gene regulation leading to the genesis of cancer. The biochemical mechanisms by which nuclear oncoproteins act remain unknown. Recently, an oncogene, v-jun, was found to share homology with the DNA binding domain of a yeast transcription factor, GCN4. Furthermore, GCN4 and the phorbol ester-inducible enhancer binding protein, AP-1, recognize very similar DNA sequences. The human proto-oncogene c-jun has now been isolated, and the deduced amino acid sequence indicates more than 80 percent identity with v-jun. Expression of cloned c-jun in bacteria produced a protein with sequence-specific DNA binding properties identical to AP-1. Antibodies raised against two distinct peptides derived from v-jun reacted specifically with human AP-1. In addition, partial amino acid sequence of purified AP-1 revealed tryptic peptides in common with the c-jun protein. The structural and functional similarities between the c-jun product and the enhancer binding protein suggest that AP-1 may be encoded by c-jun. These findings demonstrate that the proto-oncogene product of c-jun interacts directly with specific target DNA sequences to regulate gene expression, and therefore it may now be possible to identify genes under the control of c-jun that affect cell growth and neoplasia.


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K S Ullman, J P Northrop, A Admon, and G R Crabtree (1993)
Genes & Dev. 7, 188-196
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Drosophila C/EBP: a tissue-specific DNA-binding protein required for embryonic development..
P Rorth and D J Montell (1992)
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E Bossy-Wetzel, R Bravo, and D Hanahan (1992)
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T Abel, R Bhatt, and T Maniatis (1992)
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DNA bending by Fos and Jun: the flexible hinge model.
T. Kerppola and T Curran (1991)
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HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA.
M. Hoekstra, R. Liskay, A. Ou, A. DeMaggio, D. Burbee, and F Heffron (1991)
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Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
T. Wilson, T. Fahrner, M Johnston, and J Milbrandt (1991)
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Ubiquitin Hybrid Protein Gene Expression During Human Colon Cancer Progression.
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Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases..
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Efficient transformation of chicken embryo fibroblasts by c-Jun requires structural modification in coding and noncoding sequences..
T J Bos, F S Monteclaro, F Mitsunobu, A R Ball, C H Chang, T Nishimura, and P K Vogt (1990)
Genes & Dev. 4, 1677-1687
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The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer..
P D Gregor, M Sawadogo, and R G Roeder (1990)
Genes & Dev. 4, 1730-1740
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Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity..
Y F Hu, B Luscher, A Admon, N Mermod, and R Tjian (1990)
Genes & Dev. 4, 1741-1752
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Transcription factor interactions: selectors of positive or negative regulation from a single DNA element.
M. Diamond, J. Miner, S. Yoshinaga, and K. Yamamoto (1990)
Science 249, 1266-1272
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Redox regulation of fos and jun DNA-binding activity in vitro.
C Abate, L Patel, F. Rauscher 3rd, and T Curran (1990)
Science 249, 1157-1161
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Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells..
P A Ney, B P Sorrentino, K T McDonagh, and A W Nienhuis (1990)
Genes & Dev. 4, 993-1006
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The Drosophila Fos-related AP-1 protein is a developmentally regulated transcription factor..
K K Perkins, A Admon, N Patel, and R Tjian (1990)
Genes & Dev. 4, 822-834
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Nerve growth factor regulates tyrosine hydroxylase gene transcription through a nucleoprotein complex that contains c-Fos..
E Gizang-Ginsberg and E B Ziff (1990)
Genes & Dev. 4, 477-491
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Different requirements for formation of Jun: Jun and Jun: Fos complexes..
T Smeal, P Angel, J Meek, and M Karin (1989)
Genes & Dev. 3, 2091-2100
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Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1..
R P Fisher, M A Parisi, and D A Clayton (1989)
Genes & Dev. 3, 2202-2217
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Interleukin-1 costimulatory activity on the interleukin-2 promoter via AP-1.
K Muegge, T. Williams, J Kant, M Karin, R Chiu, A Schmidt, U Siebenlist, H. Young, and S. Durum (1989)
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Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer..
S L Forsburg and L Guarente (1989)
Genes & Dev. 3, 1166-1178
   Abstract »    PDF »
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
P. Mitchell and R Tjian (1989)
Science 245, 371-378
   Abstract »    PDF »
Co-localization of elements required for phorbol ester stimulation and glucocorticoid repression of proliferin gene expression..
J C Mordacq and D I Linzer (1989)
Genes & Dev. 3, 760-769
   Abstract »    PDF »
Fos-Jun interaction: mutational analysis of the leucine zipper domain of both proteins..
L J Ransone, J Visvader, P Sassone-Corsi, and I M Verma (1989)
Genes & Dev. 3, 770-781
   Abstract »    PDF »
AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells.
L. Bernstein and N. Colburn (1989)
Science 244, 566-569
   Abstract »    PDF »
RNA-binding proteins as developmental regulators..
R J Bandziulis, M S Swanson, and G Dreyfuss (1989)
Genes & Dev. 3, 431-437
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Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
R Turner and R Tjian (1989)
Science 243, 1689-1694
   Abstract »    PDF »
Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains.
R Gentz, F. Rauscher 3rd, C Abate, and T Curran (1989)
Science 243, 1695-1699
   Abstract »    PDF »
Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins..
W S Moye-Rowley, K D Harshman, and C S Parker (1989)
Genes & Dev. 3, 283-292
   Abstract »    PDF »
A FOS protein is present in a complex that binds a negative regulator of MYC..
N Hay, M Takimoto, and J M Bishop (1989)
Genes & Dev. 3, 293-303
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Growth factors and membrane depolarization activate distinct programs of early response gene expression: dissociation of fos and jun induction..
D P Bartel, M Sheng, L F Lau, and M E Greenberg (1989)
Genes & Dev. 3, 304-313
   Abstract »    PDF »
The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein..
W F Odenwald, J Garbern, H Arnheiter, E Tournier-Lasserve, and R A Lazzarini (1989)
Genes & Dev. 3, 158-172
   Abstract »    PDF »



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Science. ISSN 0036-8075 (print), 1095-9203 (online)