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Science 27 November 1987:
Vol. 238. no. 4831, pp. 1288 - 1292
DOI: 10.1126/science.2446390
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Articles

Science, Vol 238, Issue 4831, 1288-1292
Copyright © 1987 by American Association for the Advancement of Science

articles

A G protein directly regulates mammalian cardiac calcium channels

A Yatani, J Codina, Y Imoto, JP Reeves, L Birnbaumer, and AM Brown

Department of Physiology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030.

A possible direct effect of guanine nucleotide binding (G) proteins on calcium channels was examined in membrane patches excised from guinea pig cardiac myocytes and bovine cardiac sarcolemmal vesicles incorporated into planar lipid bilayers. The guanosine triphosphate analog, GTP gamma S, prolonged the survival of excised calcium channels independently of the presence of adenosine 3',5'-monophosphate (cAMP), adenosine triphosphate, cAMP-activated protein kinase, and the protein kinase C activator tetradecanoyl phorbol acetate. A specific G protein, activated Gs, or its alpha subunit, purified from the plasma membranes of human erythrocytes, prolonged the survival of excised channels and stimulated the activity of incorporated channels. Thus, in addition to regulating calcium channels indirectly through activation of cytoplasmic kinases, G proteins can regulate calcium channels directly. Since they also directly regulate a subset of potassium channels, G proteins are now known to directly gate two classes of membrane ion channels.


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