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Science 27 November 1987:
Vol. 238. no. 4831, pp. 1275 - 1278
DOI: 10.1126/science.3685978
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Articles

Science, Vol 238, Issue 4831, 1275-1278
Copyright © 1987 by American Association for the Advancement of Science

articles

Acylation of proteins with myristic acid occurs cotranslationally

C Wilcox, JS Hu, and EN Olson

Department of Biochemistry and Molecular Biology, University of Texas, M.D. Anderson Hospital and Tumor Institute at Houston 77030.

Several proteins of viral and cellular origin are acylated with myristic acid early during their biogenesis. To investigate the possibility that myristylation occurred cotranslationally, the BC3H1 muscle cell line, which contains a broad array of myristylated proteins, was pulse-labeled with [3H]myristic acid. Nascent polypeptide chains covalently associated with transfer RNA were isolated subsequently by ion-exchange chromatography. [3H]Myristate was attached to nascent chains through an amide linkage and was identified by thin-layer chromatography after its release from nascent chains by acid methanolysis. Inhibition of cellular protein synthesis with puromycin resulted in cessation of [3H]myristate-labeling of nascent chains, in agreement with the dependence of this modification on protein synthesis in vivo. These data represent a direct demonstration that myristylation of proteins is a cotranslational modification.


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