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Science 13 November 1987:
Vol. 238. no. 4829, pp. 960 - 963
DOI: 10.1126/science.2823388
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Articles

Science, Vol 238, Issue 4829, 960-963
Copyright © 1987 by American Association for the Advancement of Science

articles

Transposon tagging and molecular analysis of the maize regulatory locus opaque-2

RJ Schmidt, FA Burr, and B Burr

Biology Department, Brookhaven National Laboratory, Upton, NY 11973.

Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Wild-Type Opaque2 and Defective opaque2 Polypeptides Form Complexes in Maize Endosperm Cells and Bind the Opaque2-Zein Target Site.
F. Gavazzi, B. Lazzari, P. Ciceri, E. Gianazza, and A. Viotti (2007)
Plant Physiology 145, 933-945
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Identification and Characterization of Endoplasmic Reticulum-Associated Degradation Proteins Differentially Affected by Endoplasmic Reticulum Stress.
M. E. Kirst, D. J. Meyer, B. C. Gibbon, R. Jung, and R. S. Boston (2005)
Plant Physiology 138, 218-231
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A New Opaque Variant of Maize by a Single Dominant RNA-Interference-Inducing Transgene.
G. Segal, R. Song, and J. Messing (2003)
Genetics 165, 387-397
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Arabidopsis ABI5 Subfamily Members Have Distinct DNA-Binding and Transcriptional Activities.
S. Y. Kim, J. Ma, P. Perret, Z. Li, and T. L. Thomas (2002)
Plant Physiology 130, 688-697
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Oliver Nelson and Quality Protein Maize.
J. F. Crow and J. Kermicle (2002)
Genetics 160, 819-821
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Molecular Variation and F1 Performance among Strains of the Sweet Corn Inbred P39.
W.F. Tracy, L.E. Talbert, and J.T. Gerdes (2000)
Crop Sci. 40, 1763-1768
   Abstract »    Full Text »    PDF »
Specific Combinations of Zein Genes and Genetic Backgrounds Influence the Transcription of the Heavy-Chain Zein Genes in Maize opaque-2 Endosperms.
P. Ciceri, S. Castelli, M. Lauria, B. Lazzari, A. Genga, L. Bernard, M. Sturaro, and A. Viotti (2000)
Plant Physiology 124, 451-460
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The Activity of the Maize Opaque2 Transcriptional Activator Is Regulated Diurnally.
P. Ciceri, F. Locatelli, A. Genga, A. Viotti, and R. J. Schmidt (1999)
Plant Physiology 121, 1321-1327
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Characterization of a Maize Tonoplast Aquaporin Expressed in Zones of Cell Division and Elongation.
F. Chaumont, F. Barrieu, E. M. Herman, and M. J. Chrispeels (1998)
Plant Physiology 117, 1143-1152
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Analysis of the Methylation Pattern of the Maize Opaque-2 (O2) Promoter and in Vitro Binding Studies Indicate That the O2 B-Zip Protein and Other Endosperm Factors Can Bind to Methylated Target Sequences.
V. Rossi, M. Motto, and L. Pellegrini (1997)
J. Biol. Chem. 272, 13758-13765
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An arginine to lysine substitution in the bZIP domain of an opaque-2 mutant in maize abolishes specific DNA binding..
M J Aukerman, R J Schmidt, B Burr, and F A Burr (1991)
Genes & Dev. 5, 310-320
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Genetically Engineering Plants for Crop Improvement.
C. S. GASSER and R. T. FRALEY (1989)
Science 244, 1293-1299
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A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis.
K. A. Feldmann, M. D. Marks, M. L. Christianson, and R. S. Quatrano (1989)
Science 243, 1351-1354
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Plants: novel developmental processes.
R. Goldberg (1988)
Science 240, 1460-1467
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Use of the Transposon Ac as a Gene-Searching Engine in the Maize Genome.
M. Cowperthwaite, W. Park, Z. Xu, X. Yan, S. C. Maurais, and H. K. Dooner (2002)
PLANT CELL 14, 713-726
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Science. ISSN 0036-8075 (print), 1095-9203 (online)