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Science 6 November 1987:
Vol. 238. no. 4828, pp. 804 - 807
DOI: 10.1126/science.238.4828.804
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Articles

Discovery of Transposable Element Activity Among Progeny of Tissue Culture—Derived Maize Plants

VIRGINIA M. PESCHKE 1, RONALD L. PHILLIPS 1, and BURLE G. GENGENBACH 1

1 Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108.

Tissue culture-derived plants of many species have often been observed to possess both genetic and cytogenetic abnormalities. A high frequency of structurally altered chromosomes in maize (Zea mays L.) plants regenerated from tissue culture led to the prediction that newly activated transposable elements could be detected in regenerated plants. Testcrosses of 1200 progeny from 301 regenerated maize plants confirmed that ten regenerated plants from two independent embryo cell lines contained an active Actransposable element. No active Ac elements were present in the explant sources. Recovery of transposable element activity in regenerated plants indicates that some tissue culture—derived genetic variability may be the result of insertion or excision of transposable elements, or both.

Submitted on January 28, 1987
Accepted on July 31, 1987


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Close Split of Sorghum and Maize Genome Progenitors.
Z. Swigonova, J. Lai, J. Ma, W. Ramakrishna, V. Llaca, J. L. Bennetzen, and J. Messing (2004)
Genome Res. 14, 1916-1923
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Genome System Architecture and Natural Genetic Engineering in Evolution.
J. A. SHAPIRO (1999)
Ann. N.Y. Acad. Sci. 870, 23-35
   Abstract »    Full Text »    PDF »
The Transposition Frequency of Tag1 Elements Is Increased in Transgenic Arabidopsis Lines.
A. M. Bhatt, C. Lister, N. Crawford, and C. Dean (1998)
PLANT CELL 10, 427-434
   Abstract »    Full Text »    PDF »
Identification of a mobile endogenous transposon in Arabidopsis thaliana.
Y. Tsay, M. Frank, T Page, C Dean, and N. Crawford (1993)
Science 260, 342-344
   Abstract »    PDF »
Phenotypic diversity mediated by the maize transposable elements Ac and Spm.
S. Wessler (1988)
Science 242, 399-405
   Abstract »    PDF »
Sequence, Regulation, and Evolution of the Maize 22-kD alpha Zein Gene Family.
R. Song, V. Llaca, E. Linton, and J. Messing (2001)
Genome Res. 11, 1817-1825
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)