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Science 6 November 1987:
Vol. 238. no. 4828, pp. 800 - 802
DOI: 10.1126/science.3313729

Articles

Science, Vol 238, Issue 4828, 800-802
Copyright © 1987 by American Association for the Advancement of Science


articles

Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line

TM Folks, J Justement, A Kinter, CA Dinarello, and AS Fauci

Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

A model system for cytokine-induced up-regulation of human immunodeficiency virus type 1 (HIV-1) expression in chronically infected promonocyte clones was established. The parent promonocyte cell line U937 was chronically infected with HIV-1 and from this line a clone, U1, was derived. U1 showed minimal constitutive expression of HIV-1, but virus expression was markedly up-regulated by a phytohemagglutinin-induced supernatant containing multiple cytokines and by recombinant granulocyte/macrophage colony-stimulating factor alone. Recombinant interleukin-1 (IL-1), IL-2, interferon-gamma, and tumor necrosis factor-alpha did not up-regulate virus expression. Concomitant with the cytokine-induced up-regulation of HIV-1, expression of membrane-bound IL-1 beta was selectively induced in U1 in the absence of induction of other surface membrane proteins. This cytokine up-regulation of IL-1 beta was not seen in the uninfected parent U937 cell line. These studies have implications for the understanding of the mechanism of progression from a latent or low-level HIV-1 infection to a productive infection with resulting immunosuppression. In addition, this model can be used to delineate the potential mechanisms whereby HIV-1 infection regulates cellular gene expression.


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S. Emiliani, W. Fischle, M. Ott, C. Van Lint, C. A. Amella, and E. Verdin (1998)
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C. Giovannangeli, S. Diviacco, V. Labrousse, S. Gryaznov, P. Charneau, and C. Helene (1997)
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CD8+ T-cell-derived soluble factor(s), but not beta -chemokines RANTES, MIP-1alpha , and MIP-1beta , suppress HIV-1 replication in monocyte/macrophages.
H. Moriuchi, M. Moriuchi, C. Combadiere, P. M. Murphy, and A. S. Fauci (1996)
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A. L. Kinter, M. Ostrowski, D. Goletti, A. Oliva, D. Weissman, K. Gantt, E. Hardy, R. Jackson, L. Ehler, and A. S. Fauci (1996)
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Interferon-alpha but not AZT suppresses HIV expression in chronically infected cell lines.
G Poli, J. Orenstein, A Kinter, T. Folks, and A. Fauci (1989)
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Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow.
T. Folks, S. Kessler, J. Orenstein, J. Justement, E. Jaffe, and A. Fauci (1988)
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Cytokines alter production of HIV-1 from primary mononuclear phagocytes.
Y Koyanagi, W. O'Brien, J. Zhao, D. Golde, J. Gasson, and I. Chen (1988)
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The human immunodeficiency virus: infectivity and mechanisms of pathogenesis.
A. Fauci (1988)
Science 239, 617-622
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