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Science 6 November 1987:
Vol. 238. no. 4828, pp. 791 - 793
DOI: 10.1126/science.3118465
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Articles

Science, Vol 238, Issue 4828, 791-793
Copyright © 1987 by American Association for the Advancement of Science

articles

Early restriction of the human antibody repertoire

HW Schroeder Jr, JL Hillson, and RM Perlmutter

Howard Hughes Medical Institute, University of Washington, Seattle 98195.

Diversification of the antibody repertoire in mammals results from a series of apparently random somatically propagated gene rearrangement and mutational events. Nevertheless, it is well known that the adult repertoire of antibody specificities is acquired in a developmentally programmed fashion. As previously shown, rearrangement of the gene segments encoding the heavy-chain variable regions (VH) of mouse antibodies is also developmentally ordered: the number of VH gene segments rearranged in B lymphocytes of fetal mice is small but increased progressively after birth. In this report, human fetal B-lineage cells were also shown to rearrange a highly restricted set of VH gene segments. In a sample of heavy-chain transcripts from a 130-day human fetus the most frequently expressed human VH element proved to be closely related to the VH element most frequently expressed in murine fetal B-lineage cells. These observations are important in understanding the development of immunocompetence.


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