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Science 6 November 1987:
Vol. 238. no. 4828, pp. 788 - 791
DOI: 10.1126/science.3672126
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Articles

Science, Vol 238, Issue 4828, 788-791
Copyright © 1987 by American Association for the Advancement of Science

articles

A novel thyroid hormone receptor encoded by a cDNA clone from a human testis library

D Benbrook and M Pfahl

Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.

The c-erbA gene belongs to a multigene family that encodes transcriptional regulatory proteins including the v-erbA oncogene product, steroid hormone receptors, and the vitamin D3 receptor. A v-erbA DNA probe encoding the DNA-binding region of the v-erbA protein was used to screen a human complementary DNA testis library. One of the clones isolated, erbA-T-1, was found to encode a 490-amino acid protein (erbA-T). The erbA-T polypeptide shows high homology with the proteins encoded by both the chicken c-erbA and the human c-erbA-beta genes but is most closely related to the chicken gene. The chicken c-erbA and the human c-erbA-beta genes encode high-affinity receptors for thyroid hormone, and here it is shown that the erbA-T protein binds specifically to 3,5,3'-triiodo-L-thyronine with a dissociation constant of 3.8 +/- 0.2 x 10(-10) M. These data imply that more than one thyroid hormone receptor exists in humans and that these receptors might have different tissue- and gene-activating specificities.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Neonatal Hypothyroidism Alters the Localization of Gap Junctional Protein Connexin 43 in the Testis and Messenger RNA Levels in the Epididymis of the Rat.
N. St-Pierre, J. Dufresne, A. A. Rooney, and D. G. Cyr (2003)
Biol Reprod 68, 1232-1240
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A Prospective Controlled Study of the Impact of Hyperthyroidism on Reproductive Function in Males.
G. E. Krassas, N. Pontikides, V. Deligianni, and K. Miras (2002)
J. Clin. Endocrinol. Metab. 87, 3667-3671
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Feedback on Hypothalamic TRH Transcription Is Dependent on Thyroid Hormone Receptor N Terminus.
H. Guissouma, S. M. Dupre, N. Becker, E. Jeannin, I. Seugnet, B. Desvergne, and B. A. Demeneix (2002)
Mol. Endocrinol. 16, 1652-1666
   Abstract »    Full Text »    PDF »
Nuclear Corepressor and Silencing Mediator of Retinoic and Thyroid Hormone Receptors Corepressor Expression Is Incompatible with T3-Dependent TRH Regulation.
N. Becker, I. Seugnet, H. Guissouma, S. M. Dupre, and B. A. Demeneix (2001)
Endocrinology 142, 5321-5331
   Abstract »    Full Text »    PDF »
Assessment of Mechanisms of Thyroid Hormone Action in Mouse Leydig Cells: Regulation of the Steroidogenic Acute Regulatory Protein, Steroidogenesis, and Luteinizing Hormone Receptor Function.
P. R. Manna, J. Kero, M. Tena-Sempere, P. Pakarinen, D. M. Stocco, and I. T. Huhtaniemi (2001)
Endocrinology 142, 319-331
   Abstract »    Full Text »    PDF »
Ontogenetic Pattern of Thyroid Hormone Receptor Expression in the Human Testis.
E. A. Jannini, A. Crescenzi, N. Rucci, E. Screponi, E. Carosa, A. De Matteis, E. Macchia, G. d’Amati, and M. D’Armiento (2000)
J. Clin. Endocrinol. Metab. 85, 3453-3457
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Developmental Expression of Thyroid Hormone Receptors in the Rat Testis.
J. J. Buzzard, J. R. Morrison, M. K. O'Bryan, Q. Song, and N. G. Wreford (2000)
Biol Reprod 62, 664-669
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Ligand-dependent Heterodimerization of Thyroid Hormone Receptor and Retinoid X Receptor.
T. Kakizawa, T. Miyamoto, A. Kaneko, H. Yajima, K. Ichikawa, and K. Hashizume (1997)
J. Biol. Chem. 272, 23799-23804
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Similar Ligand-induced Conformational Changes of Thyroid Hormone Receptors Regulate Homo- and Heterodimeric Functions.
I. Bendik and M. Pfahl (1995)
J. Biol. Chem. 270, 3107-3114
   Abstract »    Full Text »    PDF »
Phosphorylation of the v-erbA protein is required for its function as an oncogene..
C Glineur, M Zenke, H Beug, and J Ghysdael (1990)
Genes & Dev. 4, 1663-1676
   Abstract »    PDF »
Molecular cloning of human and rat complementary DNA encoding androgen receptors.
C. Chang, J Kokontis, and S. Liao (1988)
Science 240, 324-326
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)