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Science 6 November 1987:
Vol. 238. no. 4828, pp. 773 - 777
DOI: 10.1126/science.3313728
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Articles

Science, Vol 238, Issue 4828, 773-777
Copyright © 1987 by American Association for the Advancement of Science

articles

Left-handed DNA in vivo

A Jaworski, WT Hsieh, JA Blaho, JE Larson, and RD Wells

Department of Biochemistry, School of Medicine, University of Alabama, Birmingham 35294.

Left-handed DNA is shown to exist and elicit a biological response in Escherichia coli. A plasmid encoding the gene for a temperature-sensitive Eco RI methylase (MEco RI) was cotransformed with different plasmids containing inserts that had varying capacities to form left-handed helices or cruciforms with a target Eco RI site in the center or at the ends of the inserts. Inhibition of methylation in vivo was found for the stable inserts with the longest left-handed (presumably Z) helices. In vitro methylation with the purified MEco RI agreed with the results in vivo. Supercoil-induced changes in the structure of the primary helix in vitro provided confirmation that left-handed helices were responsible for this behavior. The presence in vivo of left-handed inserts elicits specific deletions and plasmid incompatibilities in certain instances.


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Genetic Instabilities in (CTG{middle dot}CAG) Repeats Occur by Recombination.
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Cloning, Characterization, and Properties of Seven Triplet Repeat DNA Sequences.
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The Biology of Left-handed Z-DNA.
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Analysis of Left-handed Z-DNA Formation in Short d(CG)[IMAGE] Sequences in Escherichia coli and Halobacterium halobium Plasmids.
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Stabilization of Z DNA in vivo by localized supercoiling.
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Gene Conversion (Recombination) Mediates Expansions of CTG{middle dot}CAG Repeats.
J. P. Jakupciak and R. D. Wells (2000)
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Contextual Equilibrium Effects in DNA Molecules.
R. Goobes and A. Minsky (2001)
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PKD1 Unusual DNA Conformations Are Recognized by Nucleotide Excision Repair.
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