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Science 30 October 1987:
Vol. 238. no. 4827, pp. 672 - 675
DOI: 10.1126/science.2823384
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Articles

Science, Vol 238, Issue 4827, 672-675
Copyright © 1987 by American Association for the Advancement of Science

articles

An M2 muscarinic receptor subtype coupled to both adenylyl cyclase and phosphoinositide turnover

A Ashkenazi, JW Winslow, EG Peralta, GL Peterson, MI Schimerlik, DJ Capon, and J Ramachandran

Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080.

To investigate whether a particular receptor subtype can be coupled to multiple effector systems, recombinant M2 muscarinic receptors were expressed in cells lacking endogenous receptor. The muscarinic agonist carbachol both inhibited adenylyl cyclase and stimulated phosphoinositide hydrolysis. The stimulation of phosphoinositide hydrolysis was significantly less efficient and more dependent on receptor levels than the inhibition of adenylyl cyclase. Both responses were mediated by guanine nucleotide binding proteins, as evidenced by their inhibition by pertussis toxin; the more efficiently coupled adenylyl cyclase response was significantly more sensitive. Thus, individual subtypes of a given receptor are capable of regulating multiple effector pathways.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)