Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Click Me!

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 23 October 1987:
Vol. 238. no. 4826, pp. 545 - 548
DOI: 10.1126/science.2821625
Prev | Table of Contents | Next

Articles

Science, Vol 238, Issue 4826, 545-548
Copyright © 1987 by American Association for the Advancement of Science

articles

Germline organization of the murine T cell receptor beta-chain genes

HS Chou, CA Nelson, SA Godambe, DD Chaplin, and DY Loh

Howard Hughes Medical Institute, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

The complete germline organization of the beta-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (V beta) genes are clustered within 250 kilobases of DNA 5' to the constant region (C beta) genes. These V beta genes share the same transcriptional orientation as the diversity (D beta), joining (J beta), and C beta genes, which implies that chromosomal deletion is the mechanism for most V beta to D beta-J beta rearrangements. Within this V beta cluster, the distance between the most proximal V beta gene and the D beta-J beta-C beta cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V beta and D beta, relative to that between D beta and J beta, may have significant implications for the ordered rearrangement of the T cell receptor beta-chain genes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distinct Control of the Frequency and Allelic Exclusion of the V{beta} Gene Rearrangement at the TCR{beta} Locus.
P. Sieh and J. Chen (2001)
J. Immunol. 167, 2121-2129
   Abstract »    Full Text »    PDF »
Oligoclonal T Cell Expansions in Atherosclerotic Lesions of Apolipoprotein E-Deficient Mice.
G. Paulsson, X. Zhou, E. Tornquist, and G. K. Hansson (2000)
Arterioscler. Thromb. Vasc. Biol. 20, 10-17
   Abstract »    Full Text »    PDF »
Caprine T-Cell Receptor Variable {beta}-Chain (TCRV{beta}) Repertoire Analysis and Potential Applications in Cowdriosis Immune Response Studies.
G. OBEXER-RUFF, A. FLURI, W. HEIN, S. LAZARY, E. PETERHANS, and G. BERTONI (1998)
Ann. N.Y. Acad. Sci. 849, 321-326
   Abstract »    Full Text »    PDF »
Biochemical and Functional Analyses of Chromatin Changes at the TCR-{beta} Gene Locus During CD4-CD8- to CD4+CD8+ Thymocyte Differentiation.
S. Chattopadhyay, C. E. Whitehurst, F. Schwenk, and J. Chen (1998)
J. Immunol. 160, 1256-1267
   Abstract »    Full Text »    PDF »
A transcriptional enhancer 3' of C beta 2 in the T cell receptor beta locus.
S McDougall, C. Peterson, and K Calame (1988)
Science 241, 205-208
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)