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Science 23 October 1987:
Vol. 238. no. 4826, pp. 527 - 530
DOI: 10.1126/science.2443980
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Articles

Science, Vol 238, Issue 4826, 527-530
Copyright © 1987 by American Association for the Advancement of Science

articles

Model substrates for an RNA enzyme

WH McClain, C Guerrier-Takada, and S Altman

Department of Bacteriology, University of Wisconsin, Madison 53706.

M1 RNA, the catalytic RNA subunit of Escherichia coli ribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3' terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)