Related Content
Search Google Scholar for:
|
|
Science 23 October 1987:
Vol. 238. no. 4826, pp. 504 - 509
DOI: 10.1126/science.3116672
|
|
Articles
Science, Vol 238, Issue 4826, 504-509
Copyright © 1987 by American Association for the Advancement of Science
Clathrin requirement for normal growth of yeast
SK Lemmon
and
EW Jones
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213.
Clathrin-coated membranes and coated vesicles take part in the selective transfer of proteins between different subcellular compartments of eukaryotic cells. To allow assessment of the role of clathrin in vesicular transport, genetic analysis of the clathrin heavy chain gene (CHC1) in Saccharomyces cerevisiae was initiated. The complete heavy chain gene was cloned, and the effects of deletion of this gene were studied. The null mutation (chc1-delta) is lethal unless a suppressor of clathrin deficiency (scd1) is present. Even in the presence of the suppressor gene, mutants lacking the clathrin heavy chain grow slowly, are genetically unstable, are morphologically abnormal, and show loss of or reduction in several yeast functions. These results indicate that clathrin is required for normal growth of yeast, and, therefore, most likely, for growth of all eukaryotic cells.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Novel Function of Clathrin Light Chain in Promoting Endocytic Vesicle Formation.
- T. M. Newpher, F.-Z. Idrissi, M. I. Geli, and S. K. Lemmon (2006)
Mol. Biol. Cell
17, 4343-4352
| Abstract »
| Full Text »
| PDF »
- Highly Cooperative Control of Endocytosis by Clathrin.
- H. S. Moskowitz, C. T. Yokoyama, and T. A. Ryan (2005)
Mol. Biol. Cell
16, 1769-1776
| Abstract »
| Full Text »
| PDF »
- Targeted Chemical Disruption of Clathrin Function in Living Cells.
- H. S. Moskowitz, J. Heuser, T. E. McGraw, and T. A. Ryan (2003)
Mol. Biol. Cell
14, 4437-4447
| Abstract »
| Full Text »
| PDF »
- The Synaptojanin-like Protein Inp53/Sjl3 Functions with Clathrin in a Yeast TGN-to-Endosome Pathway Distinct from the GGA Protein-dependent Pathway.
- S.-A. Ha, J. Torabinejad, D. B. DeWald, M. R. Wenk, L. Lucast, P. De Camilli, R. A. Newitt, R. Aebersold, and S. F. Nothwehr (2003)
Mol. Biol. Cell
14, 1319-1333
| Abstract »
| Full Text »
| PDF »
- Controlled Elimination of Clathrin Heavy-Chain Expression in DT40 Lymphocytes.
- F. R. Wettey, S. F. C. Hawkins, A. Stewart, J. P. Luzio, J. C. Howard, and A. P. Jackson (2002)
Science
297, 1521-1525
| Abstract »
| Full Text »
| PDF »
- Scd5p and Clathrin Function Are Important for Cortical Actin Organization, Endocytosis, and Localization of Sla2p in Yeast.
- K. R. Henry, K. D'Hondt, J. Chang, T. Newpher, K. Huang, R. T. Hudson, H. Riezman, and S. K. Lemmon (2002)
Mol. Biol. Cell
13, 2607-2625
| Abstract »
| Full Text »
| PDF »
- Synthetic Genetic Interactions With Temperature-Sensitive Clathrin in Saccharomyces cerevisiae: Roles for Synaptojanin-Like Inp53p and Dynamin-Related Vps1p in Clathrin-Dependent Protein Sorting at the trans-Golgi Network.
- E. S. Bensen, G. Costaguta, and G. S. Payne (2000)
Genetics
154, 83-97
| Abstract »
| Full Text »
- Involvement of Long Chain Fatty Acid Elongation in the Trafficking of Secretory Vesicles in Yeast.
- D. David, S. Sundarababu, and J. E. Gerst (1998)
J. Cell Biol.
143, 1167-1182
| Abstract »
| Full Text »
| PDF »
- Ovarian- and Somatic-specific Transcripts of the Mosquito Clathrin Heavy Chain Gene Generated by Alternative 5'-Exon Splicing and Polyadenylation.
- V. A. Kokoza and A. S. Raikhel (1997)
J. Biol. Chem.
272, 1164-1170
| Abstract »
| Full Text »
| PDF »
- Novel functions of clathrin light chains: clathrin heavy chain trimerization is defective in light chain-deficient yeast.
- K. Huang, L Gullberg, K. Nelson, C. Stefan, K Blumer, and S. Lemmon (1997)
J. Cell Sci.
110, 899-910
| Abstract »
| PDF »
- Clathrin: a role in the intracellular retention of a Golgi membrane protein.
- G. Payne and R Schekman (1989)
Science
245, 1358-1365
| Abstract »
| PDF »
- Living with clathrin: its role in intracellular membrane traffic.
- F. Brodsky (1988)
Science
242, 1396-1402
| Abstract »
| PDF »
- Yeast: an experimental organism for modern biology.
- D Botstein and G. Fink (1988)
Science
240, 1439-1443
| Abstract »
| PDF »
|
|
