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Science 25 September 1987:
Vol. 237. no. 4822, pp. 1605 - 1608
DOI: 10.1126/science.3306923
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Articles

Science, Vol 237, Issue 4822, 1605-1608
Copyright © 1987 by American Association for the Advancement of Science

articles

An in vitro neurite-promoting antigen functions in axonal regeneration in vivo

AW Sandrock Jr and WD Matthew

The function of the neurite growth-promoting antigen INO has been tested in an in vivo neurite regeneration system, the rat iris. The sympathetic innervation of the irides was removed by a single systemic injection of 6-hydroxydopamine. The subsequent regeneration of sympathetic axons into the iris of one eye bathed by the INO antibody, which inhibits neurite growth in vitro, was compared with the regrowth of sympathetic axons into the iris of the animal's other eye, which contained control antibody. Antibodies were released within the eye by implanted hybridoma cells. Neurite regeneration was measured by assaying [3H]norepinephrine uptake into freshly explained irides. The blockage of the function of the INO antigen by the antibody resulted in a decreased rate of axonal regeneration, thus suggesting the involvement of the INO antigen in the process of neurite regeneration in vivo.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Differential effects of glycosaminoglycans on neurite outgrowth from hippocampal and thalamic neurones.
I Fernaud-Espinosa, M Nieto-Sampedro, and P Bovolenta (1994)
J. Cell Sci. 107, 1437-1448
   Abstract »    PDF »
Regulation of expression of fibronectin and its receptor, alpha 5 beta 1, during development and regeneration of peripheral nerve.
F Lefcort, K Venstrom, J. McDonald, and L. Reichardt (1992)
Development 116, 767-782
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)