Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Click Me!

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 September 1987:
Vol. 237. no. 4821, pp. 1476 - 1479
DOI: 10.1126/science.3629250
Prev | Table of Contents | Next

Articles

Science, Vol 237, Issue 4821, 1476-1479
Copyright © 1987 by American Association for the Advancement of Science

articles

Expression of an exogenous growth hormone gene by transplantable human epidermal cells

Morgan JR, Y Barrandon, H Green, and RC Mulligan

Retrovirus-mediated gene transfer was used to introduce a recombinant human growth hormone gene into cultured human keratinocytes. The transduced keratinocytes secreted biologically active growth hormone into the culture medium. When grafted as an epithelial sheet onto athymic mice, these cultured keratinocytes reconstituted an epidermis that was similar in appearance to that resulting from normal cells, but from which human growth hormone could be extracted. Transduced epidermal cells may prove to be a general vehicle for the delivery of gene products by means of grafting.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Review Paper: Burn Coverage Technologies: Current Concepts and Future Directions.
C. Pereira, W. Gold, and D. Herndon (2007)
J Biomater Appl 22, 101-121
   PDF »
Bioengineering Obesity: The Skinny on Leptin Delivery.
M. Hickey (2005)
Endocrinology 146, 4165-4166
   Full Text »    PDF »
Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo.
W. Pfutzner, A. Terunuma, C. L. Tock, E. K. Snead, T. M. Kolodka, M. M. Gottesman, L. Taichman, and J. C. Vogel (2002)
PNAS 99, 13096-13101
   Abstract »    Full Text »    PDF »
In Vivo Assessment of Gene Delivery to Keratinocytes by Lentiviral Vectors.
U. Kuhn, A. Terunuma, W. Pfutzner, R. A. Foster, and J. C. Vogel (2002)
J. Virol. 76, 1496-1504
   Abstract »    Full Text »    PDF »
A cutaneous gene therapy approach to human leptin deficiencies: correction of the murine ob/ob phenotype using leptin-targeted keratinocyte grafts.
F. LARCHER, M. DEL RIO, F. SERRANO, J. C. SEGOVIA, A. RAMIREZ, A. MEANA, A. PAGE, J. L. ABAD, M. A. GONZALEZ, J. BUEREN, et al. (2001)
FASEB J 15, 1529-1538
   Abstract »    Full Text »    PDF »
Keratinocyte growth factor induces hyperproliferation and delays differentiation in a skin equivalent model system.
S. T. ANDREADIS, K. E. HAMOEN, M. L. YARMUSH, and J. R. MORGAN (2001)
FASEB J 15, 898-906
   Abstract »    Full Text »    PDF »
Correction of the coagulation defect in hemophilia A mice through factor VIII expression in skin.
S. S. Fakharzadeh, Y. Zhang, R. Sarkar, and H. H. Kazazian Jr (2000)
Blood 95, 2799-2805
   Abstract »    Full Text »    PDF »
Regulation of the Spatial Organization of Mesenchymal Connective Tissue : Effects of Cell-Associated versus Released Isoforms of Platelet-Derived Growth Factor.
S. A. Eming, M. L. Yarmush, G. G. Krueger, and J. R. Morgan (1999)
Am. J. Pathol. 154, 281-289
   Abstract »    Full Text »    PDF »
Evidence for keratinocyte stem cells in vitro: Long term engraftment and persistence of transgene expression from retrovirus-transduced keratinocytes.
T. M. Kolodka, J. A. Garlick, and L. B. Taichman (1998)
PNAS 95, 4356-4361
   Abstract »    Full Text »    PDF »
Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene therapy.
X. Wang, S. Zinkel, K. Polonsky, and E. Fuchs (1997)
PNAS 94, 219-226
   Abstract »    Full Text »    PDF »
Long-term expression of human growth hormone (hGH) in mice containing allogeneic yolk sac cell derived neovascular implants expressing hGH.
Y Wei, J Li, and T. Wagner (1996)
Stem Cells 14, 232-238
   Abstract »
The basic science of gene therapy.
R. Mulligan (1993)
Science 260, 926-932
   Abstract »    PDF »
Systemic delivery of human growth hormone by injection of genetically engineered myoblasts.
J Dhawan, L. Pan, G. Pavlath, M. Travis, A. Lanctot, and H. Blau (1991)
Science 254, 1509-1512
   Abstract »    PDF »
New methods of drug delivery.
R Langer (1990)
Science 249, 1527-1533
   Abstract »    PDF »
High-level recombinant gene expression in rabbit endothelial cells transduced by retroviral vectors.
J. Zwiebel, S. Freeman, P. Kantoff, K Cornetta, U. Ryan, and W. Anderson (1989)
Science 243, 220-222
   Abstract »    PDF »
Grafting genetically modified cells to the damaged brain: restorative effects of NGF expression.
M. Rosenberg, T Friedmann, R. Robertson, M Tuszynski, J. Wolff, X. Breakefield, and F. Gage (1988)
Science 242, 1575-1578
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)