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Science 4 September 1987:
Vol. 237. no. 4819, pp. 1217 - 1219
DOI: 10.1126/science.3498213
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Articles

Science, Vol 237, Issue 4819, 1217-1219
Copyright © 1987 by American Association for the Advancement of Science

articles

Measuring the human T cell receptor gamma-chain locus

WM Strauss, T Quertermous, and JG Seidman

The human T cell receptor gamma locus, including eleven variable-region, five joining-region, and two constant-region segments, is contained in 160 kilobases. During T cell somatic development these genes undergo rearrangement by deletion of the sequences separating the variable and joining regions. The molecular map of this locus was completely defined by deletion mapping and restriction mapping. Restriction fragments were resolved by standard agarose electrophoresis and field inversion electrophoresis. These studies demonstrate that the deletions in this locus, which occur during the formation of a functional T cell receptor gamma-chain gene, range from 50 to 145 kilobases in length. These studies also provide a structural basis for understanding the development of the gamma-chain peptide repertoire, and extends the potential of the emerging pulsed-field electrophoretic technology.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Induction of Germline Transcription in the Human TCR{{gamma}} Locus by STAT5.
H.-C. Lee, S.-K. Ye, T. Honjo, and K. Ikuta (2001)
J. Immunol. 167, 320-326
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Extensive junctional diversity of rearranged human T cell receptor delta genes.
S Hata, K Satyanarayana, P Devlin, H Band, J McLean, J. Strominger, M. Brenner, and M. Krangel (1988)
Science 240, 1541-1544
   Abstract »    PDF »


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