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Science 28 August 1987:
Vol. 237. no. 4818, pp. 1039 - 1041
DOI: 10.1126/science.3616625
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Articles

Science, Vol 237, Issue 4818, 1039-1041
Copyright © 1987 by American Association for the Advancement of Science

articles

The raf oncogene is associated with a radiation-resistant human laryngeal cancer

U Kasid, A Pfeifer, RR Weichselbaum, A Dritschilo, and GE Mark

In order to identify the genetic factors associated with the radiation-resistant human laryngeal carcinoma cell line (SQ-20B), tumor cell DNA was transfected into NIH/3T3 cells. A high incidence (six out of six) of raf sequences was found in transfected NIH/3T3 clones and the tumorigenic potential of SQ-20B DNA could be linked to genomic fragments that represent most of the kinase domain of human c-raf-1. An apparently unaltered 3.5-kilobase pair (kb) human c-raf transcript was identified in SQ-20B cells but was not observed in the transfected NIH/3T3 cell clones. Two new transcripts (4.2 kb and 2.6 kb) were found in tumorigenic clones; the large transcript was missing in a very poorly tumorigenic clone. Cytogenetic analysis indicated that the normal autosomes of chromosome 3 were absent in SQ-20B karyotypes and had formed apparently stable marker chromosomes. Unlike the recipient NIH/3T3 cell line, 30 percent of the transformed clone-1 metaphases had minute and double-minute chromosomes representative of amplified DNA sequences. The frequency of the c-raf-1 identification by NIH/3T3 transfection of SQ-20B DNA suggests the presence of some genetic abnormality within this locus.


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Raf: A Strategic Target for Therapeutic Development Against Cancer.
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Selective Inhibition of Ras, Phosphoinositide 3 Kinase, and Akt Isoforms Increases the Radiosensitivity of Human Carcinoma Cell Lines.
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W. D. Merritt, M. C. Weissler, B. F. Turk, and T. M. Gilmer (1990)
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Effect of antisense c-raf-1 on tumorigenicity and radiation sensitivity of a human squamous carcinoma.
U Kasid, A Pfeifer, T Brennan, M Beckett, R. Weichselbaum, A Dritschilo, and G. Mark (1989)
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Oncogenes in radioresistant, noncancerous skin fibroblasts from a cancer-prone family.
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