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Science 28 August 1987:
Vol. 237. no. 4818, pp. 1007 - 1012
DOI: 10.1126/science.2887035
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Articles

Science, Vol 237, Issue 4818, 1007-1012
Copyright © 1987 by American Association for the Advancement of Science

articles

Homeo domain of the yeast repressor alpha 2 is a sequence-specific DNA-binding domain but is not sufficient for repression

MN Hall and AD Johnson

The alpha 2 protein, the product of the MAT alpha 2 gene, is a regulator of cell type in the yeast Saccharomyces cerevisiae. It represses transcription of a group of cell type-specific genes by binding to an operator located upstream of each target gene. Fifteen in-frame deletions within the coding region of the MAT alpha 2 gene were constructed. The deletion alleles were examined for phenotypes conferred in vivo, and the encoded mutant proteins were assayed for ability to bind specifically to the operator in vitro. This analysis has revealed that the sequence-specific DNA-binding domain of alpha 2 is located within a region of 68 amino acids. This region of alpha 2 has significant homology with the homeo domain, a conserved sequence found in the products of several Drosophila homeotic and segmentation genes. In addition, there is a class of mutant alpha 2 proteins that binds tightly and specifically to the operator in vitro, but fails to repress transcription in vivo.


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A Complex Composed of Tup1 and Ssn6 Represses Transcription in Vitro.
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The Yeast Homeodomain Protein MATalpha 2 Shows Extended DNA binding Specificity in Complex with Mcm1.
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Genetic characterization of the role of the two HOX proteins, Proboscipedia and Sex Combs Reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila melanogaster.
A Percival-Smith, J Weber, E Gilfoyle, and P Wilson (1997)
Development 124, 5049-5062
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Functional domains in the Deformed protein.
A Zhu and M. Kuziora (1996)
Development 122, 1577-1587
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A homeo domain protein lacking specific side chains of helix 3 can still bind DNA and direct transcriptional repression..
A K Vershon, Y Jin, and A D Johnson (1995)
Genes & Dev. 9, 182-192
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The WD repeats of Tup1 interact with the homeo domain protein alpha 2..
K Komachi, M J Redd, and A D Johnson (1994)
Genes & Dev. 8, 2857-2867
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The carboxy-terminal tail of the homeo domain protein alpha 2 is required for function with a second homeo domain protein..
A Mak and A D Johnson (1993)
Genes & Dev. 7, 1862-1870
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The mouse Hox-1.3 gene is functionally equivalent to the Drosophila Sex combs reduced gene..
J J Zhao, R A Lazzarini, and L Pick (1993)
Genes & Dev. 7, 343-354
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Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4..
S Y Roth, M Shimizu, L Johnson, M Grunstein, and R T Simpson (1992)
Genes & Dev. 6, 411-425
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High-affinity binding sites for the Deformed protein are required for the function of an autoregulatory enhancer of the Deformed gene..
M Regulski, S Dessain, N McGinnis, and W McGinnis (1991)
Genes & Dev. 5, 278-286
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The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain..
C P Verrijzer, A J Kal, and P C van der Vliet (1990)
Genes & Dev. 4, 1964-1974
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The E2 trans-activator can act as a repressor by interfering with a cellular transcription factor..
A Stenlund and M R Botchan (1990)
Genes & Dev. 4, 123-136
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DNA binding by proteins.
R Schleif (1988)
Science 241, 1182-1187
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Expression, modification, and localization of the fushi tarazu protein in Drosophila embryos..
H M Krause, R Klemenz, and W J Gehring (1988)
Genes & Dev. 2, 1021-1036
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Flexibility of the yeast alpha 2 repressor enables it to occupy the ends of its operator, leaving the center free..
R T Sauer, D L Smith, and A D Johnson (1988)
Genes & Dev. 2, 807-816
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Pair--rule segmentation genes regulate the expression of the homeotic selector gene, Deformed.
T. Jack, M. Regulski, and W. McGinnis (1988)
Genes & Dev. 2, 635-651
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Yeast HAP2 and HAP3: transcriptional activators in a heteromeric complex.
S Hahn and L Guarente (1988)
Science 240, 317-321
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