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Science 28 August 1987:
Vol. 237. no. 4818, pp. 1007 - 1012
DOI: 10.1126/science.2887035
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Articles

Science, Vol 237, Issue 4818, 1007-1012
Copyright © 1987 by American Association for the Advancement of Science

articles

Homeo domain of the yeast repressor alpha 2 is a sequence-specific DNA-binding domain but is not sufficient for repression

MN Hall and AD Johnson

The alpha 2 protein, the product of the MAT alpha 2 gene, is a regulator of cell type in the yeast Saccharomyces cerevisiae. It represses transcription of a group of cell type-specific genes by binding to an operator located upstream of each target gene. Fifteen in-frame deletions within the coding region of the MAT alpha 2 gene were constructed. The deletion alleles were examined for phenotypes conferred in vivo, and the encoded mutant proteins were assayed for ability to bind specifically to the operator in vitro. This analysis has revealed that the sequence-specific DNA-binding domain of alpha 2 is located within a region of 68 amino acids. This region of alpha 2 has significant homology with the homeo domain, a conserved sequence found in the products of several Drosophila homeotic and segmentation genes. In addition, there is a class of mutant alpha 2 proteins that binds tightly and specifically to the operator in vitro, but fails to repress transcription in vivo.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Homeodomain Leucine Zipper Class I Genes in Arabidopsis. Expression Patterns and Phylogenetic Relationships.
E. Henriksson, A. S.B. Olsson, H. Johannesson, H. Johansson, J. Hanson, P. Engstrom, and E. Soderman (2005)
Plant Physiology 139, 509-518
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A Complex Composed of Tup1 and Ssn6 Represses Transcription in Vitro.
M. J. Redd, M. B. Arnaud, and A. D. Johnson (1997)
J. Biol. Chem. 272, 11193-11197
   Abstract »    Full Text »    PDF »
The Yeast Homeodomain Protein MATalpha 2 Shows Extended DNA binding Specificity in Complex with Mcm1.
H. Zhong and A. K. Vershon (1997)
J. Biol. Chem. 272, 8402-8409
   Abstract »    Full Text »    PDF »
Genetic characterization of the role of the two HOX proteins, Proboscipedia and Sex Combs Reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila melanogaster.
A Percival-Smith, J Weber, E Gilfoyle, and P Wilson (1997)
Development 124, 5049-5062
   Abstract »    PDF »
Functional domains in the Deformed protein.
A Zhu and M. Kuziora (1996)
Development 122, 1577-1587
   Abstract »    PDF »
A homeo domain protein lacking specific side chains of helix 3 can still bind DNA and direct transcriptional repression..
A K Vershon, Y Jin, and A D Johnson (1995)
Genes & Dev. 9, 182-192
   Abstract »    PDF »
The WD repeats of Tup1 interact with the homeo domain protein alpha 2..
K Komachi, M J Redd, and A D Johnson (1994)
Genes & Dev. 8, 2857-2867
   Abstract »    PDF »
The carboxy-terminal tail of the homeo domain protein alpha 2 is required for function with a second homeo domain protein..
A Mak and A D Johnson (1993)
Genes & Dev. 7, 1862-1870
   Abstract »    PDF »
The mouse Hox-1.3 gene is functionally equivalent to the Drosophila Sex combs reduced gene..
J J Zhao, R A Lazzarini, and L Pick (1993)
Genes & Dev. 7, 343-354
   Abstract »    PDF »
Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4..
S Y Roth, M Shimizu, L Johnson, M Grunstein, and R T Simpson (1992)
Genes & Dev. 6, 411-425
   Abstract »    PDF »
High-affinity binding sites for the Deformed protein are required for the function of an autoregulatory enhancer of the Deformed gene..
M Regulski, S Dessain, N McGinnis, and W McGinnis (1991)
Genes & Dev. 5, 278-286
   Abstract »    PDF »
The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain..
C P Verrijzer, A J Kal, and P C van der Vliet (1990)
Genes & Dev. 4, 1964-1974
   Abstract »    PDF »
The E2 trans-activator can act as a repressor by interfering with a cellular transcription factor..
A Stenlund and M R Botchan (1990)
Genes & Dev. 4, 123-136
   Abstract »    PDF »
DNA binding by proteins.
R Schleif (1988)
Science 241, 1182-1187
   Abstract »    PDF »
Expression, modification, and localization of the fushi tarazu protein in Drosophila embryos..
H M Krause, R Klemenz, and W J Gehring (1988)
Genes & Dev. 2, 1021-1036
   Abstract »    PDF »
Flexibility of the yeast alpha 2 repressor enables it to occupy the ends of its operator, leaving the center free..
R T Sauer, D L Smith, and A D Johnson (1988)
Genes & Dev. 2, 807-816
   Abstract »    PDF »
Pair--rule segmentation genes regulate the expression of the homeotic selector gene, Deformed.
T. Jack, M. Regulski, and W. McGinnis (1988)
Genes & Dev. 2, 635-651
   Abstract »    PDF »
Yeast HAP2 and HAP3: transcriptional activators in a heteromeric complex.
S Hahn and L Guarente (1988)
Science 240, 317-321
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)