Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 August 1987:
Vol. 237. no. 4816, pp. 777 - 779
DOI: 10.1126/science.3649921
Prev | Table of Contents | Next

Articles

Science, Vol 237, Issue 4816, 777-779
Copyright © 1987 by American Association for the Advancement of Science

articles

Heparin promotes the inactivation of antithrombin by neutrophil elastase

RE Jordan, J Kilpatrick, and RM Nelson

Heparin is an acceleratory cofactor for antithrombin, a circulating inhibitor of blood coagulation enzymes. The presence of heparin on blood vessel walls is believed to contribute to the nonthrombogenic properties of those surfaces. In apparent opposition to this function, heparin was found to greatly accelerate the in vitro inactivation of antithrombin by neutrophil elastase. Inactivation rates in solution were potentiated several hundredfold by specific heparin fractions with anticoagulant activity. Although the data suggest that a heparin-antithrombin complex is essential for the inactivation by elastase to occur, the enzyme itself interacts tightly with heparin. These results suggest a mechanism which, if operating in vivo, could lead to a localized neutralization of the anticoagulant function of heparin at the endothelial surface.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Characterization of the Conformational Alterations, Reduced Anticoagulant Activity, and Enhanced Antiangiogenic Activity of Prelatent Antithrombin.
B. Richard, R. Swanson, S. Schedin-Weiss, B. Ramirez, G. Izaguirre, P. G. W. Gettins, and S. T. Olson (2008)
J. Biol. Chem. 283, 14417-14429
   Abstract »    Full Text »    PDF »
Mechanism by Which Exosites Promote the Inhibition of Blood Coagulation Proteases by Heparin-activated Antithrombin.
G. Izaguirre, R. Swanson, S. M. Raja, A. R. Rezaie, and S. T. Olson (2007)
J. Biol. Chem. 282, 33609-33622
   Abstract »    Full Text »    PDF »
Influence of modified ultrafiltration on coagulation, fibrinolysis and blood loss in adult cardiac surgery.
R. G. Leyh, C. Bartels, E. Joubert-Hubner, J. F.M. Bechtel, and H. H. Sievers (2001)
Eur. J. Cardiothorac. Surg. 19, 145-151
   Abstract »    Full Text »    PDF »
Polymorphonuclear leukocyte activation and hemostasis in patients with essential thrombocythemia and polycythemia vera.
A. Falanga, M. Marchetti, V. Evangelista, A. Vignoli, M. Licini, M. Balicco, S. Manarini, G. Finazzi, C. Cerletti, and T. Barbui (2000)
Blood 96, 4261-4266
   Abstract »    Full Text »    PDF »
Neutrophil Activation and Hemostatic Changes in Healthy Donors Receiving Granulocyte Colony-Stimulating Factor.
A. Falanga, M. Marchetti, V. Evangelista, S. Manarini, E. Oldani, S. Giovanelli, M. Galbusera, C. Cerletti, and T. Barbui (1999)
Blood 93, 2506-2514
   Abstract »    Full Text »    PDF »
Heparin-dependent Modification of the Reactive Center Arginine of Antithrombin and Consequent Increase in Heparin Binding Affinity.
R. N. Pike, J. Potempa, R. Skinner, H. L. Fitton, W. T. McGraw, J. Travis, M. Owen, L. Jin, and R. W. Carrell (1997)
J. Biol. Chem. 272, 19652-19655
   Abstract »    Full Text »    PDF »
Human Neutrophil Elastase Activates Human Factor V but Inactivates Thrombin-Activated Human Factor V.
J. A. Samis, M. Garrett, R. P. Manuel, M. E. Nesheim, and A. R. Giles (1997)
Blood 90, 1065-1074
   Abstract »    Full Text »    PDF »
The Suprapharmacologic Dosing of Antithrombin Concentrate for Staphylococcus Aureus-Induced Disseminated Intravascular Coagulation in Guinea Pigs: Substantial Reduction in Mortality and Morbidity.
C. M. Kessler, Z. Tang, H. M. Jacobs, and L. M. Szymanski (1997)
Blood 89, 4393-4401
   Abstract »    Full Text »    PDF »
Neutrophil Activation in Acute Renal Failure and Sepsis.
W. H. Horl, R. M. Schafer, M. Horl, and A. Heidland (1990)
Arch Surg 125, 651-654
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)