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Science 14 August 1987:
Vol. 237. no. 4816, pp. 770 - 775
DOI: 10.1126/science.2441471
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Articles

Science, Vol 237, Issue 4816, 770-775
Copyright © 1987 by American Association for the Advancement of Science

articles

Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila

BL Tempel, DM Papazian, TL Schwarz, YN Jan, and LY Jan

Potassium currents are crucial for the repolarization of electrically excitable membranes, a role that makes potassium channels a target for physiological modifications that alter synaptic efficacy. The Shaker locus of Drosophila is thought to encode a K+ channel. The sequence of two complementary DNA clones from the Shaker locus is reported here. The sequence predicts an integral membrane protein of 70,200 daltons containing seven potential membrane-spanning sequences. In addition, the predicted protein is homologous to the vertebrate sodium channel in a region previously proposed to be involved in the voltage-dependent activation of the Na+ channel. These results support the hypothesis that Shaker encodes a structural component of a voltage-dependent K+ channel and suggest a conserved mechanism for voltage activation.


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