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Science 7 August 1987:
Vol. 237. no. 4815, pp. 645 - 647
DOI: 10.1126/science.3110954
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Articles

Science, Vol 237, Issue 4815, 645-647
Copyright © 1987 by American Association for the Advancement of Science

articles

Eosinophils cocultured with endothelial cells have increased survival and functional properties

ME Rothenberg, WF Owen Jr, DS Silberstein, RJ Soberman, KF Austen, and RL Stevens

Human peripheral blood eosinophils, cells often associated with allergic and parasitic diseases, were maintained in vitro for at least 14 days when they were cocultured with bovine endothelial cells and for at least 7 days when cultured with either bovine or human endothelial cell-derived conditioned medium. The cocultured eosinophils became hypodense and generated about three times as much leukotriene C4 upon activation with calcium ionophore and killed about three times as many antibody-coated larvae of Schistosoma mansoni as freshly isolated normodense eosinophils. That these cells can be maintained in vitro by coculture with endothelial cells, and the surprising finding that the cocultured eosinophils have biochemical, cytotoxic, and density properties similar to those of eosinophils in patients with allergic and other disorders, will facilitate investigation of the regulation and role of these cells in health and disease.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inhibitory receptor gp49B regulates eosinophil infiltration during allergic inflammation.
H. H. Norris, M. E. Peterson, C. C. Stebbins, B. W. McConchie, V. G. Bundoc, S. Trivedi, M. G. Hodges, R. M. Anthony, J. F. Urban Jr, E. O. Long, et al. (2007)
J. Leukoc. Biol. 82, 1531-1541
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Analysis of Eosinophil Turnover In Vivo Reveals Their Active Recruitment to and Prolonged Survival in the Peritoneal Cavity.
C. Ohnmacht, A. Pullner, N. van Rooijen, and D. Voehringer (2007)
J. Immunol. 179, 4766-4774
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Activation of human eosinophils via P2 receptors: novel findings and future perspectives.
D. Ferrari, A. la Sala, E. Panther, J. Norgauer, F. Di Virgilio, and M. Idzko (2006)
J. Leukoc. Biol. 79, 7-15
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Eosinophils Maintain Their Capacity to Signal and Release Eosinophil Cationic Protein Upon Repetitive Stimulation with the Same Agonist.
H.-U. Simon, M. Weber, E. Becker, Y. Zilberman, K. Blaser, and F. Levi-Schaffer (2000)
J. Immunol. 165, 4069-4075
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Syk-deficient eosinophils show normal interleukin-5-mediated differentiation, maturation, and survival but no longer respond to Fcgamma R activation.
E. Lach-Trifilieff, K. Menear, E. Schweighoffer, V. L. J. Tybulewicz, and C. Walker (2000)
Blood 96, 2506-2510
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The Effect of Transendothelial Migration on Eosinophil Function.
H. Yamamoto, J. B. Sedgwick, R. F. Vrtis, and W. W. Busse (2000)
Am. J. Respir. Cell Mol. Biol. 23, 379-388
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Pharmacology of the Eosinophil.
M. A. Giembycz and M. A. Lindsay (1999)
Pharmacol. Rev. 51, 213-340
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Eosinophilia.
M. E. Rothenberg (1998)
N. Engl. J. Med. 338, 1592-1600
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Science. ISSN 0036-8075 (print), 1095-9203 (online)