Related Content
Search Google Scholar for:
|
|
Science 7 August 1987:
Vol. 237. no. 4815, pp. 635 - 639
DOI: 10.1126/science.3299708
|
|
Articles
Science, Vol 237, Issue 4815, 635-639
Copyright © 1987 by American Association for the Advancement of Science
erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation
VN Rao,
TS Papas,
and
ES Reddy
The avian acute leukemia virus E26 induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. Recently, a novel gene named erg, closely related to the v-ets oncogene, was identified in human COLO 320 cells and the nucleotide sequence of its approximately 5.0-kilobase transcript, erg 1 was determined. In the present study, the nucleotide sequence of the alternatively spliced transcript, erg 2, was found to differ from erg 1 by a splicing event that causes a coding frameshift near the amino terminus, resulting in an additional 99-amino acid insertion at the amino-terminus. Expression of complementary DNAs for the two transcripts in vitro resulted in synthesis of polypeptides of approximately 41 and 52 kilodaltons, suggesting the use of alternative translation initiation codons in the case of erg proteins. The erg gene was localized by somatic cell genetic analysis to human chromosome 21. It is proposed that alternative sites of splicing and polyadenylation, together with alternative sites of translation initiation, allow the synthesis of two related polypeptides from a single erg gene transcriptional unit.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Transcription Factor ERG Variants and Functional Diversification of Chondrocytes during Limb Long Bone Development.
- M. Iwamoto, Y. Higuchi, E. Koyama, M. Enomoto-Iwamoto, K. Kurisu, H. Yeh, W. R. Abrams, J. Rosenbloom, and M. Pacifici (2000)
J. Cell Biol.
150, 27-40
| Abstract »
| Full Text »
| PDF »
- Dual Transforming Activities of the FUS (TLS)-ERG Leukemia Fusion Protein Conferred by Two N-Terminal Domains of FUS (TLS).
- H. Ichikawa, K. Shimizu, R. Katsu, and M. Ohki (1999)
Mol. Cell. Biol.
19, 7639-7650
| Abstract »
| Full Text »
| PDF »
- Prf, a novel Ets family protein that binds to the PU.1 binding motif, is specifically expressed in restricted stages of B cell development.
- S.-i. Hashimoto, H. Nishizumi, R. Hayashi, A. Tsuboi, F. Nagawa, T. Takemori, and H. Sakano (1999)
Int. Immunol.
11, 1423-1429
| Abstract »
| Full Text »
| PDF »
- Requirement of a GT Box (Sp1 Site) and Two Ets Binding Sites for Vascular Endothelial Cadherin Gene Transcription.
- S. Gory, J. Dalmon, M.-H. Prandini, T. Kortulewski, Y. de Launoit, and P. Huber (1998)
J. Biol. Chem.
273, 6750-6755
| Abstract »
| Full Text »
| PDF »
- Consistent Detection of TLS/FUS-ERG Chimeric Transcripts in Acute Myeloid Leukemia With t(16; 21)(p11; q22) and Identification of a Novel Transcript.
- X.-T. Kong, K. Ida, H. Ichikawa, K. Shimizu, M. Ohki, N. Maseki, Y. Kaneko, M. Sako, Y. Kobayashi, A. Tojou, et al. (1997)
Blood
90, 1192-1199
| Abstract »
| Full Text »
| PDF »
- Characterization of a gp91-phox Promoter Element That Is Required for Interferon [IMAGE]-induced Transcription.
- E. A. Eklund and D. G. Skalnik (1995)
J. Biol. Chem.
270, 8267-8273
| Abstract »
| Full Text »
| PDF »
- Erythroleukemia induction by Friend murine leukemia virus: insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets-1..
- Y Ben-David, E B Giddens, K Letwin, and A Bernstein (1991)
Genes & Dev.
5, 908-918
| Abstract »
| PDF »
- Sequence-specific binding of human Ets-1 to the T cell receptor alpha gene enhancer.
- I. Ho, N. Bhat, L. Gottschalk, T Lindsten, C. Thompson, T. Papas, and J. Leiden (1990)
Science
250, 814-818
| Abstract »
| PDF »
- Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus..
- C V Gunther, J A Nye, R S Bryner, and B J Graves (1990)
Genes & Dev.
4, 667-679
| Abstract »
| PDF »
- Rapid and transient expression of Ets2 in mature macrophages following stimulation with cMGF, LPS, and PKC activators..
- K E Boulukos, P Pognonec, E Sariban, M Bailly, C Lagrou, and J Ghysdael (1990)
Genes & Dev.
4, 401-409
| Abstract »
| PDF »
- elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.
- V. Rao, K Huebner, M Isobe, A ar-Rushdi, C. Croce, and E. Reddy (1989)
Science
244, 66-70
| Abstract »
| PDF »
|
|
