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Science 10 July 1987:
Vol. 237. no. 4811, pp. 175 - 178
DOI: 10.1126/science.3110950
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Articles

Science, Vol 237, Issue 4811, 175-178
Copyright © 1987 by American Association for the Advancement of Science

articles

Detection of minimal residual cells carrying the t(14;18) by DNA sequence amplification

MS Lee, KS Chang, F Cabanillas, EJ Freireich, JM Trujillo, and SA Stass

By means of the polymerase chain reaction (PCR) technique, DNA sequences were amplified that flank the crossover sites of a characteristic chromosomal translocation for follicular lymphomas, t(14;18)(q32;q21). This technique permitted the detection of cells carrying the t(14;18) hybrid DNA sequences at a dilution of 1:100,000. The remission marrow and blood samples of a patient with follicular lymphoma and the t(14;18) failed to show any abnormality by morphological examination and conventional Southern blot analysis. However, the t(14;18) hybrid DNA sequences were detected by the PCR technique. Thus, this technique is a highly sensitive tool to detect minimal residual cells carrying the t(14;18) and has the potential to identify a subpopulation of patients with subclinical disease.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)