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Science 26 June 1987:
Vol. 236. no. 4809, pp. 1666 - 1671
DOI: 10.1126/science.3037693
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Articles

Science, Vol 236, Issue 4809, 1666-1671
Copyright © 1987 by American Association for the Advancement of Science

articles

A promoter with an internal regulatory domain is part of the origin of replication in BPV-1

A Stenlund, GL Bream, and MR Botchan

Extrachromosomal elements that are stably maintained at a constant copy number through cell doublings are a good model system for the study of the regulation of DNA replication in higher eukaryotes. Previous studies have defined both cis and trans functions required for the regulated plasmid replication of the bovine papilloma virus in stably transformed cells. Here, a sequence known to be a cis-dominant element of the replication origin of the plasmid is shown to contain a promoter for transcription. Both in vitro and in vivo assays have been used to define this promoter and show that a sequence located just 3' to the transcriptional start site is required for activity. This DNA sequence element, which has been defined through deletions, coincides with a binding site for a cellular factor and is also required for a functional origin of replication. Possible models for how a transcription factor may play a role in the regulation of DNA replication are discussed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cloning and Characterization of the Mouse Histone Deacetylase-2 Gene.
Y. Zeng, C.-M. Tang, Y.-L. Yao, W.-M. Yang, and E. Seto (1998)
J. Biol. Chem. 273, 28921-28930
   Abstract »    Full Text »    PDF »
Cellular Transcription Factors Enhance Herpes Simplex Virus Type 1 oriS-Dependent DNA Replication.
A. T. Nguyen-Huynh and P. A. Schaffer (1998)
J. Virol. 72, 3635-3645
   Abstract »    Full Text »    PDF »
The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex..
N Dostatni, P F Lambert, R Sousa, J Ham, P M Howley, and M Yaniv (1991)
Genes & Dev. 5, 1657-1671
   Abstract »    PDF »
The E2 trans-activator can act as a repressor by interfering with a cellular transcription factor..
A Stenlund and M R Botchan (1990)
Genes & Dev. 4, 123-136
   Abstract »    PDF »
A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes..
S Passmore, R Elble, and B K Tye (1989)
Genes & Dev. 3, 921-935
   Abstract »    PDF »
Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters..
K A Jones, P A Luciw, and N Duchange (1988)
Genes & Dev. 2, 1101-1114
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)