Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 June 1987:
Vol. 236. no. 4807, pp. 1448 - 1453
DOI: 10.1126/science.3296194
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4807, 1448-1453
Copyright © 1987 by American Association for the Advancement of Science

articles

A physical map of the Escherichia coli K12 genome

CL Smith, JG Econome, A Schutt, S Klco, and CR Cantor

A physical map of a genome is the structure of its DNA. Construction of such a map is a first step in the complete characterization of that DNA. The restriction endonuclease Not I cuts the genome of Escherichia coli K12 into 22 DNA fragments ranging from 20 kilobases (20,000 base pairs) to 1000 kilobases. These can be separated by pulsed field gel electrophoresis. The order of the fragments in the genome was determined from available E. coli genetic information and analysis of partial digest patterns. The resulting ordered set of fragments is a macrorestriction map. This map facilitates genetic and molecular studies on E. coli, and its construction serves as a model for further endeavors on larger genomes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phylogenetic Characterization of Two Novel Commensal Bacteria Involved with Innate Immune Homeostasis in Drosophila melanogaster.
S. W. Roh, Y.-D. Nam, H.-W. Chang, K.-H. Kim, M.-S. Kim, J.-H. Ryu, S.-H. Kim, W.-J. Lee, and J.-W. Bae (2008)
Appl. Envir. Microbiol. 74, 6171-6177
   Abstract »    Full Text »    PDF »
Genome Plasticity and ori-ter Rebalancing in Salmonella typhi.
G.-R. Liu, W.-Q. Liu, R. N. Johnston, K. E. Sanderson, S.-X. Li, and S.-L. Liu (2006)
Mol. Biol. Evol. 23, 365-371
   Abstract »    Full Text »    PDF »
Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans.
J. Lin, R. Qi, C. Aston, J. Jing, T. S. Anantharaman, B. Mishra, O. White, M. J. Daly, K. W. Minton, J. C. Venter, et al. (1999)
Science 285, 1558-1562
   Abstract »    Full Text »
Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status.
C.-L. Lin, J.-C. Lee, S.-S. Chen, H. Alan Wood, M.-L. Li, C.-F. Li, and Y.-C. Chao (1999)
J. Virol. 73, 128-139
   Abstract »    Full Text »    PDF »
The marine bacterium Pseudoalteromonas haloplanktis has a complex genome structure composed of two separate genetic units..
B D Lanoil, L M Ciuffetti, and S J Giovannoni (1996)
Genome Res. 6, 1160-1169
   Abstract »    PDF »
tau Couples the Leading- and Lagging-strand Polymerases at the Escherichia coli DNA Replication Fork.
S. Kim, H. G. Dallmann, C. S. McHenry, and K. J. Marians (1996)
J. Biol. Chem. 271, 21406-21412
   Abstract »    Full Text »    PDF »
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
D. Daniels, G Plunkett 3rd, V Burland, and F. Blattner (1992)
Science 257, 771-778
   Abstract »    PDF »
Cleaving yeast and Escherichia coli genomes at a single site.
M Koob and W Szybalski (1990)
Science 250, 271-273
   Abstract »    PDF »
Orchestrating the Human Genome Project.
C. Cantor (1990)
Science 248, 49-51
   Abstract »    PDF »
A common language for physical mapping of the human genome.
M Olson, L Hood, C Cantor, and D Botstein (1989)
Science 245, 1434-1435
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)