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Science 5 June 1987:
Vol. 236. no. 4806, pp. 1305 - 1308
DOI: 10.1126/science.3035716
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Articles

Science, Vol 236, Issue 4806, 1305-1308
Copyright © 1987 by American Association for the Advancement of Science

articles

Derivation of clones close to met by preparative field inversion gel electrophoresis

F Michiels, M Burmeister, and H Lehrach

The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of the cystic fibrosis gene: chromosome walking and jumping.
J. Rommens, M. Iannuzzi, B Kerem, M. Drumm, G Melmer, M Dean, R Rozmahel, J. Cole, D Kennedy, N Hidaka, et al. (1989)
Science 245, 1059-1065
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