Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 5 June 1987:
Vol. 236. no. 4806, pp. 1293 - 1295
DOI: 10.1126/science.3589663
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4806, 1293-1295
Copyright © 1987 by American Association for the Advancement of Science

articles

Selective inactivation of influenza C esterase: a probe for detecting 9-O-acetylated sialic acids

EA Muchmore and A Varki

The influenza C virus (INF-C) hemagglutinin recognizes 9-O-acetyl-N-acetylneuraminic acid. The same protein contains the receptor-destroying enzyme (RDE), which is a 9-O-acetyl-esterase. The RDE was inactivated by the serine esterase inhibitor di-isopropyl fluorophosphate (DFP). [3H]DFP-labeling localized the active site to the heavy chain of the glycoprotein. DFP did not alter the hemagglutination or fusion properties of the protein, but markedly decreased infectivity of the virus, demonstrating that the RDE is important for primary infection. Finally, DFP-treated INF-C bound specifically and irreversibly to cells expressing 9-O-acetylated sialic acids. This provides a probe for a molecule that was hitherto very difficult to study.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and Characterization of Viral Structural Proteins of Infectious Salmon Anemia Virus.
K. Falk, V. Aspehaug, R. Vlasak, and C. Endresen (2004)
J. Virol. 78, 3063-3071
   Abstract »    Full Text »    PDF »
Genes Modulated by Expression of GD3 Synthase in Chinese Hamster Ovary Cells. EVIDENCE THAT THE Tis21 GENE IS INVOLVED IN THE INDUCTION OF GD3 9-O-ACETYLATION.
H. Satake, H. Y. Chen, and A. Varki (2003)
J. Biol. Chem. 278, 7942-7948
   Abstract »    Full Text »    PDF »
Characterization of the receptor-destroying enzyme activity from infectious salmon anaemia virus.
M. Kristiansen, M. K. Froystad, A. L. Rishovd, and T. Gjoen (2002)
J. Gen. Virol. 83, 2693-2697
   Abstract »    Full Text »    PDF »
Lysosomal and Cytosolic Sialic Acid 9-O-Acetylesterase Activities Can Be Encoded by One Gene via Differential Usage of a Signal Peptide-encoding Exon at the N Terminus.
H. Takematsu, S. Diaz, A. Stoddart, Y. Zhang, and A. Varki (1999)
J. Biol. Chem. 274, 25623-25631
   Abstract »    Full Text »    PDF »
Identification of a Coronavirus Hemagglutinin-Esterase with a Substrate Specificity Different from Those of Influenza C Virus and Bovine Coronavirus.
A. Klausegger, B. Strobl, G. Regl, A. Kaser, W. Luytjes, and R. Vlasak (1999)
J. Virol. 73, 3737-3743
   Abstract »    Full Text »
9-O-Acetylation of Sialomucins: A Novel Marker of Murine CD4 T Cells that Is Regulated during Maturation and Activation.
M. Krishna and A. Varki (1997)
J. Exp. Med. 185, 1997-2013
   Abstract »    Full Text »    PDF »
Regulation of Sialic Acid 9-O-Acetylation during the Growth and Differentiation of Murine Erythroleukemia Cells.
W.-X. Shi, R. Chammas, and A. Varki (1996)
J. Biol. Chem. 271, 31517-31525
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)