Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 May 1987:
Vol. 236. no. 4805, pp. 1116 - 1120
DOI: 10.1126/science.3576226
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4805, 1116-1120
Copyright © 1987 by American Association for the Advancement of Science

articles

Expression and properties of two types of protein kinase C: alternative splicing from a single gene

Y Ono, U Kikkawa, K Ogita, T Fujii, T Kurokawa, Y Asaoka, K Sekiguchi, K Ase, K Igarashi, and Y Nishizuka

Two complementary DNA's, encoding the complete sequences of 671 and 673 amino acids for subspecies of rat brain protein kinase C, were expressed in COS 7 cells. The complementary DNA sequence analysis predicted that the two enzymes are derived from different ways of splicing and differ from each other only in the short ranges of their carboxyl-terminal regions. Both enzymes showed typical characteristics of protein kinase C that responded to Ca2+, phospholipid, and diacylglycerol. The enzymes showed practically identical physical and kinetic properties and were indistinguishable from one of the several subspecies of protein kinase C that occurs in rat brain but not in untransfected COS 7 cells. Partial analysis of the genomic structure confirmed that these two subspecies of protein kinase C resulted indeed from alternative splicing of a single gene.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Prenatal choline availability modulates hippocampal and cerebral cortical gene expression.
T. J. Mellott, M. T. Follettie, V. Diesl, A. A. Hill, I. Lopez-Coviella, and J. K. Blusztajn (2007)
FASEB J 21, 1311-1323
   Abstract »    Full Text »    PDF »
Deletion of Protein Kinase C-{beta} Isoform In Vivo Reduces Renal Hypertrophy but Not Albuminuria in the Streptozotocin-Induced Diabetic Mouse Model.
M. Meier, J.-K. Park, D. Overheu, T. Kirsch, C. Lindschau, F. Gueler, M. Leitges, J. Menne, and H. Haller (2007)
Diabetes 56, 346-354
   Abstract »    Full Text »    PDF »
Protein Kinase C {beta} Enhances Growth and Expression of Cyclin D1 in Human Breast Cancer Cells.
H. Li and I. B. Weinstein (2006)
Cancer Res. 66, 11399-11408
   Abstract »    Full Text »    PDF »
Critical Role of Protein Kinase C {beta}II in Activation of Mast Cells by Monomeric IgE.
Y. Liu, K. Furuta, R. Teshima, N. Shirata, Y. Sugimoto, A. Ichikawa, and S. Tanaka (2005)
J. Biol. Chem. 280, 38976-38981
   Abstract »    Full Text »    PDF »
Protein Kinase C {beta}II Regulates Akt Phosphorylation on Ser-473 in a Cell Type- and Stimulus-specific Fashion.
Y. Kawakami, H. Nishimoto, J. Kitaura, M. Maeda-Yamamoto, R. M. Kato, D. R. Littman, D. J. Rawlings, and T. Kawakami (2004)
J. Biol. Chem. 279, 47720-47725
   Abstract »    Full Text »    PDF »
Antisense Targeting Protein Kinase C {alpha} and {beta}1 Inhibits Gastric Carcinogenesis.
X.-H. Jiang, S.-P. Tu, J.-T. Cui, M. C. M. Lin, H. H. X. Xia, W. M. Wong, A. O.-O. Chan, M. F. Yuen, S.-H. Jiang, S.-K. Lam, et al. (2004)
Cancer Res. 64, 5787-5794
   Abstract »    Full Text »    PDF »
Isoenzyme-specific Translocation of Protein Kinase C (PKC){beta}II and not PKC{beta}I to a Juxtanuclear Subset of Recycling Endosomes: INVOLVEMENT OF PHOSPHOLIPASE D.
K. P. Becker and Y. A. Hannun (2004)
J. Biol. Chem. 279, 28251-28256
   Abstract »    Full Text »    PDF »
Insulin Regulates Protein Kinase C{beta}II Alternative Splicing in Multiple Target Tissues: Development of a Hormonally Responsive Heterologous Minigene.
N. A. Patel, H. S. Apostolatos, K. Mebert, C. E. Chalfant, J. E. Watson, T. S. Pillay, J. Sparks, and D. R. Cooper (2004)
Mol. Endocrinol. 18, 899-911
   Abstract »    Full Text »    PDF »
The V5 Domain of Protein Kinase C Plays a Critical Role in Determining the Isoform-Specific Localization, Translocation, and Biological Function of Protein Kinase C-{delta} and -{varepsilon}.
Q. J. Wang, G. Lu, W. A. Schlapkohl, A. Goerke, C. Larsson, H. Mischak, P. M. Blumberg, and J. F. Mushinski (2004)
Mol. Cancer Res. 2, 129-140
   Abstract »    Full Text »    PDF »
A Ras activation pathway dependent on Syk phosphorylation of protein kinase C.
Y. Kawakami, J. Kitaura, L. Yao, R. W. McHenry, Y. Kawakami, A. C. Newton, S. Kang, R. M. Kato, M. Leitges, D. J. Rawlings, et al. (2003)
PNAS 100, 9470-9475
   Abstract »    Full Text »    PDF »
Upregulation of PKC genes and isozymes in cardiovascular tissues during early stages of experimental diabetes.
M. Guo, M. H. Wu, F. Korompai, and S. Y. Yuan (2003)
Physiol Genomics 12, 139-146
   Abstract »    Full Text »    PDF »
Interaction of "readthrough" acetylcholinesterase with RACK1 and PKCbeta II correlates with intensified fear-induced conflict behavior.
K. R. Birikh, E. H. Sklan, S. Shoham, and H. Soreq (2003)
PNAS 100, 283-288
   Abstract »    Full Text »    PDF »
Elevated Protein Kinase C {beta}II Is an Early Promotive Event in Colon Carcinogenesis.
Y. Gökmen-Polar, N. R. Murray, M. A. Velasco, Z. Gatalica, and A. P. Fields (2001)
Cancer Res. 61, 1375-1381
   Abstract »    Full Text »
Calcium-dependent Regulation of Cytochrome c Gene Expression in Skeletal Muscle Cells. IDENTIFICATION OF A PROTEIN KINASE C-DEPENDENT PATHWAY.
D. Freyssenet, M. Di Carlo, and D. A. Hood (1999)
J. Biol. Chem. 274, 9305-9311
   Abstract »    Full Text »    PDF »
Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCßII mRNA in vascular smooth muscle cells.
N. A. Patel, C. E. Chalfant, M. Yamamoto, J. E. Watson, D. C. Eichler, and D. R. Cooper (1999)
FASEB J 13, 103-113
   Abstract »    Full Text »
Protein kinase Cbeta regulates heterologous desensitization of thrombin receptor (PAR-1) in endothelial cells.
W. Yan, C. Tiruppathi, H. Lum, R. Qiao, and A. B. Malik (1998)
Am J Physiol Cell Physiol 274, C387-C395
   Abstract »    Full Text »    PDF »
Insulin Regulates Protein Kinase Cbeta II Expression through Enhanced Exon Inclusion in L6 Skeletal Muscle Cells. A NOVEL MECHANISM OF INSULIN- AND INSULIN-LIKE GROWTH FACTOR-I-INDUCED 5' SPLICE SITE SELECTION.
C. E. Chalfant, J. E. Watson, L. D. Bisnauth, J. B. Kang, N. Patel, L. M. Obeid, D. C. Eichler, and D. R. Cooper (1998)
J. Biol. Chem. 273, 910-916
   Abstract »    Full Text »    PDF »
Ca2+ Differentially Regulates Conventional Protein Kinase Cs' Membrane Interaction and Activation.
L. M. Keranen and A. C. Newton (1997)
J. Biol. Chem. 272, 25959-25967
   Abstract »    Full Text »    PDF »
Protein-Protein Interaction of Zinc Finger LIM Domains with Protein Kinase C.
S.'i. Kuroda, C. Tokunaga, Y. Kiyohara, O. Higuchi, H. Konishi, K. Mizuno, G. N. Gill, and U. Kikkawa (1996)
J. Biol. Chem. 271, 31029-31032
   Abstract »    Full Text »    PDF »
Protein Kinase C beta II Specifically Binds to and Is Activated by F-actin.
G. C. Blobe, D. S. Stribling, D. Fabbro, S. Stabel, and Y. A. Hannun (1996)
J. Biol. Chem. 271, 15823-15830
   Abstract »    Full Text »    PDF »
Regulation of Alternative Splicing of Protein Kinase C[IMAGE] by Insulin.
C. E. Chalfant, H. Mischak, J. E. Watson, B. C. Winkler, J. Goodnight, R. V. Farese, and D. R. Cooper (1995)
J. Biol. Chem. 270, 13326-13332
   Abstract »    Full Text »    PDF »
Protein Kinase C[IMAGE]-specific Phosphorylation of the Elongation Factor eEF-1alpha and an eEF-1alpha Peptide at Threonine 431.
K. Kielbassa, H.-J. Müller, H. E. Meyer, F. Marks, and M. Gschwendt (1995)
J. Biol. Chem. 270, 6156-6162
   Abstract »    Full Text »    PDF »
Phosphorylation of Tau Is Regulated by PKN.
T. Taniguchi, T. Kawamata, H. Mukai, H. Hasegawa, T. Isagawa, M. Yasuda, T. Hashimoto, A. Terashima, M. Nakai, Y. Ono, et al. (2001)
J. Biol. Chem. 276, 10025-10031
   Abstract »    Full Text »    PDF »
Binding Specificity for RACK1 Resides in the V5 Region of beta II Protein Kinase C.
E. G. Stebbins and D. Mochly-Rosen (2001)
J. Biol. Chem. 276, 29644-29650
   Abstract »    Full Text »    PDF »
Insulin Regulates Alternative Splicing of Protein Kinase C beta II through a Phosphatidylinositol 3-Kinase-dependent Pathway Involving the Nuclear Serine/Arginine-rich Splicing Factor, SRp40, in Skeletal Muscle Cells.
N. A. Patel, C. E. Chalfant, J. E. Watson, J. R. Wyatt, N. M. Dean, D. C. Eichler, and D. R. Cooper (2001)
J. Biol. Chem. 276, 22648-22654
   Abstract »    Full Text »    PDF »
PKCtheta II, a New Isoform of Protein Kinase C Specifically Expressed in the Seminiferous Tubules of Mouse Testis.
Y. S. Niino, T. Irie, M. Takaishi, T. Hosono, N.-h. Huh, T. Tachikawa, and T. Kuroki (2001)
J. Biol. Chem. 276, 36711-36717
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)