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Science 29 May 1987:
Vol. 236. no. 4805, pp. 1106 - 1109
DOI: 10.1126/science.3576223
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Articles

Science, Vol 236, Issue 4805, 1106-1109
Copyright © 1987 by American Association for the Advancement of Science

articles

Human amnion membrane serves as a substratum for growing axons in vitro and in vivo

GE Davis, SN Blaker, E Engvall, S Varon, M Manthorpe, and FH Gage

The epithelial cell layer of human amnion membrane can be removed while the basement membrane and stromal surfaces remain morphologically intact. Such a preparation has been used as a substratum for the in vitro culture of dissociated neurons. Embryonic motor neurons from chick ciliary ganglion attached to both surfaces but grew extensive neurites only on the basement membrane. On cross sections of rolled amnion membranes, regenerating axons of cultured neurons were guided along pathways of basement membrane that were immunoreactive with an antibody to laminin. In addition, when rolled amnion membranes were implanted into a lesion cavity between the rat septum and hippocampus, cholinergic neurons extended axons through the longitudinally oriented implant into the hippocampus. Thus, this amnion preparation can serve as a bridge to promote axonal regeneration in vivo in damaged adult brain.


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