Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 29 May 1987:
Vol. 236. no. 4805, pp. 1086 - 1091
DOI: 10.1126/science.3576222
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4805, 1086-1091
Copyright © 1987 by American Association for the Advancement of Science

articles

Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination

A De Lozanne and JA Spudich

The phenomenon of homologous recombination, which allows specific gene conversion and gene insertion, can be a powerful system for the study of eukaryotic cell biology. Data are presented demonstrating that integration of a transfected plasmid by homologous recombination occurs in the motile eukaryotic cell Dictyostelium discoideum. A plasmid carrying a G418 resistance gene and the amino terminal half of the myosin heavy chain gene was used to transfect Dictyostelium. A large fraction of the resultant G418-resistant cells had the plasmid integrated into the single genomic copy of the heavy chain gene. These cells, which fail to express the native myosin but express the myosin fragment, are defective in cytokinesis and become large and multinucleate. In spite of the absence of native myosin, these cells, termed hmm cells, exhibit many forms of cell movement, including membrane ruffling, phagocytosis, and chemotaxis. The hmm cells can aggregate but are blocked at a later stage in the Dictyostelium developmental cycle. The hmm cells revert to the wild-type phenotype. Reversion of the hmm phenotype is due to excision and loss of the transforming plasmid. The revertant cells express native myosin, are G418 sensitive, and have a normal developmental cycle. These results constitute genetic proof that the intact myosin molecule is required for cytokinesis and not for karyokinesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Regulation of Fission Yeast Myosin-II Function and Contractile Ring Dynamics by Regulatory Light-Chain and Heavy-Chain Phosphorylation.
T. E. Sladewski, M. J. Previs, and M. Lord (2009)
Mol. Biol. Cell 20, 3941-3952
   Abstract »    Full Text »    PDF »
PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells.
Md. K. Pramanik, M. Iijima, Y. Iwadate, and S. Yumura (2009)
Genes Cells 14, 821-834
   Abstract »    Full Text »    PDF »
Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
R. Nishihama, J. H. Schreiter, M. Onishi, E. A. Vallen, J. Hanna, K. Moravcevic, M. F. Lippincott, H. Han, M. A. Lemmon, J. R. Pringle, et al. (2009)
J. Cell Biol. 185, 995-1012
   Abstract »    Full Text »    PDF »
Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.
S. Asano, K. Hamao, and H. Hosoya (2009)
Genes Cells 14, 555-568
   Abstract »    Full Text »    PDF »
Visualizing myosin-actin interaction with a genetically-encoded fluorescent strain sensor.
S. Iwai and T. Q. P. Uyeda (2008)
PNAS 105, 16882-16887
   Abstract »    Full Text »    PDF »
Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions.
S. Mondal, D. Bakthavatsalam, P. Steimle, B. Gassen, F. Rivero, and A. A. Noegel (2008)
J. Cell Biol. 181, 747-760
   Abstract »    Full Text »    PDF »
Myosin IIC: A Third Molecular Motor Driving Neuronal Dynamics.
S. R. Wylie and P. D. Chantler (2008)
Mol. Biol. Cell 19, 3956-3968
   Abstract »    Full Text »    PDF »
Dictyostelium Aurora Kinase Has Properties of both Aurora A and Aurora B Kinases.
H. Li, Q. Chen, M. Kaller, W. Nellen, R. Graf, and A. De Lozanne (2008)
Eukaryot. Cell 7, 894-905
   Abstract »    Full Text »    PDF »
A novel mitosis-specific dynamic actin structure in Dictyostelium cells.
G. Itoh and S. Yumura (2007)
J. Cell Sci. 120, 4302-4309
   Abstract »    Full Text »    PDF »
The Ste20-like kinase SvkA of Dictyostelium discoideum is essential for late stages of cytokinesis.
M. Rohlfs, R. Arasada, P. Batsios, J. Janzen, and M. Schleicher (2007)
J. Cell Sci. 120, 4345-4354
   Abstract »    Full Text »    PDF »
Loss of Cell Adhesion Causes Hydrocephalus in Nonmuscle Myosin II-B-ablated and Mutated Mice.
X. Ma, J. Bao, and R. S. Adelstein (2007)
Mol. Biol. Cell 18, 2305-2312
   Abstract »    Full Text »    PDF »
The N-Terminus of Dictyostelium Scar Interacts with Abi and HSPC300 and Is Essential for Proper Regulation and Function.
D. Caracino, C. Jones, M. Compton, and C. L. Saxe III (2007)
Mol. Biol. Cell 18, 1609-1620
   Abstract »    Full Text »    PDF »
Copine A Is Required for Cytokinesis, Contractile Vacuole Function, and Development in Dictyostelium.
C. K. Damer, M. Bayeva, P. S. Kim, L. K. Ho, E. S. Eberhardt, C. I. Socec, J. S. Lee, E. A. Bruce, A. E. Goldman-Yassen, and L. C. Naliboff (2007)
Eukaryot. Cell 6, 430-442
   Abstract »    Full Text »    PDF »
Enlazin, a Natural Fusion of Two Classes of Canonical Cytoskeletal Proteins, Contributes to Cytokinesis Dynamics.
E. Octtaviani, J. C. Effler, and D. N. Robinson (2006)
Mol. Biol. Cell 17, 5275-5286
   Abstract »    Full Text »    PDF »
A Point Mutation in the SH1 Helix Alters Elasticity and Thermal Stability of Myosin II.
S. Iwai, D. Hanamoto, and S. Chaen (2006)
J. Biol. Chem. 281, 30736-30744
   Abstract »    Full Text »    PDF »
A Specific Isoform of Nonmuscle Myosin II-C Is Required for Cytokinesis in a Tumor Cell Line.
S. S. Jana, S. Kawamoto, and R. S. Adelstein (2006)
J. Biol. Chem. 281, 24662-24670
   Abstract »    Full Text »    PDF »
Phosphoinositide signaling plays a key role in cytokinesis.
C. Janetopoulos and P. Devreotes (2006)
J. Cell Biol. 174, 485-490
   Abstract »    Full Text »    PDF »
Function of the Neuron-specific Alternatively Spliced Isoforms of Nonmuscle Myosin II-B during Mouse Brain Development.
X. Ma, S. Kawamoto, J. Uribe, and R. S. Adelstein (2006)
Mol. Biol. Cell 17, 2138-2149
   Abstract »    Full Text »    PDF »
Lipoxin A4 Redistributes Myosin IIA and Cdc42 in Macrophages: Implications for Phagocytosis of Apoptotic Leukocytes.
K. Reville, J. K. Crean, S. Vivers, I. Dransfield, and C. Godson (2006)
J. Immunol. 176, 1878-1888
   Abstract »    Full Text »    PDF »
Anillin Is a Substrate of Anaphase-promoting Complex/Cyclosome (APC/C) That Controls Spatial Contractility of Myosin during Late Cytokinesis.
W.-m. Zhao and G. Fang (2005)
J. Biol. Chem. 280, 33516-33524
   Abstract »    Full Text »    PDF »
MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.
W.-m. Zhao and G. Fang (2005)
PNAS 102, 13158-13163
   Abstract »    Full Text »    PDF »
Loss of SMEK, a Novel, Conserved Protein, Suppresses mek1 Null Cell Polarity, Chemotaxis, and Gene Expression Defects.
M. C. Mendoza, F. Du, N. Iranfar, N. Tang, H. Ma, W. F. Loomis, and R. A. Firtel (2005)
Mol. Cell. Biol. 25, 7839-7853
   Abstract »    Full Text »    PDF »
Multiple Myosin II Heavy Chain Kinases: Roles in Filament Assembly Control and Proper Cytokinesis in Dictyostelium.
S. Yumura, M. Yoshida, V. Betapudi, L. S. Licate, Y. Iwadate, A. Nagasaki, T. Q.P. Uyeda, and T. T. Egelhoff (2005)
Mol. Biol. Cell 16, 4256-4266
   Abstract »    Full Text »    PDF »
Adhesion-dependent and Contractile Ring-independent Equatorial Furrowing during Cytokinesis in Mammalian Cells.
M. Kanada, A. Nagasaki, and T. Q.P. Uyeda (2005)
Mol. Biol. Cell 16, 3865-3872
   Abstract »    Full Text »    PDF »
Biological, Biochemical, and Kinetic Effects of Mutations of the Cardiomyopathy Loop of Dictyostelium Myosin II: IMPORTANCE OF ALA400.
X. Liu, S. Shu, M. Kovacs, and E. D. Korn (2005)
J. Biol. Chem. 280, 26974-26983
   Abstract »    Full Text »    PDF »
Vertebrate Nonmuscle Myosin II Isoforms Rescue Small Interfering RNA-induced Defects in COS-7 Cell Cytokinesis.
J. Bao, S. S. Jana, and R. S. Adelstein (2005)
J. Biol. Chem. 280, 19594-19599
   Abstract »    Full Text »    PDF »
Balance of actively generated contractile and resistive forces controls cytokinesis dynamics.
W. Zhang and D. N. Robinson (2005)
PNAS 102, 7186-7191
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Novel {alpha}-Kinase with a von Willebrand Factor A-like Motif Localized to the Contractile Vacuole and Golgi Complex in Dictyostelium discoideum.
V. Betapudi, C. Mason, L. Licate, and T. T. Egelhoff (2005)
Mol. Biol. Cell 16, 2248-2262
   Abstract »    Full Text »    PDF »
The Molecular Requirements for Cytokinesis.
M. Glotzer (2005)
Science 307, 1735-1739
   Abstract »    Full Text »    PDF »
Blebbistatin and blebbistatin-inactivated myosin II inhibit myosin II-independent processes in Dictyostelium.
S. Shu, X. Liu, and E. D. Korn (2005)
PNAS 102, 1472-1477
   Abstract »    Full Text »    PDF »
Anillin Binds Nonmuscle Myosin II and Regulates the Contractile Ring.
A. F. Straight, C. M. Field, and T. J. Mitchison (2005)
Mol. Biol. Cell 16, 193-201
   Abstract »    Full Text »    PDF »
A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium.
G. S. Lakshmikanth, H. M. Warrick, and J. A. Spudich (2004)
PNAS 101, 16519-16524
   Abstract »    Full Text »    PDF »
The role of myosin heavy chain phosphorylation in Dictyostelium motility, chemotaxis and F-actin localization.
P. J. Heid, D. Wessels, K. J. Daniels, D. P. Gibson, H. Zhang, E. Voss, and D. R. Soll (2004)
J. Cell Sci. 117, 4819-4835
   Abstract »    Full Text »    PDF »
The Early History of the Biochemistry of Muscle Contraction.
A. G. Szent-Gyorgyi (2004)
J. Gen. Physiol. 123, 631-641
   Full Text »    PDF »
A Point Mutation in the Motor Domain of Nonmuscle Myosin II-B Impairs Migration of Distinct Groups of Neurons.
X. Ma, S. Kawamoto, Y. Hara, and R. S. Adelstein (2004)
Mol. Biol. Cell 15, 2568-2579
   Abstract »    Full Text »    PDF »
A Novel Actin-bundling Kinesin-related Protein from Dictyostelium discoideum.
S. Iwai, A. Ishiji, I. Mabuchi, and K. Sutoh (2004)
J. Biol. Chem. 279, 4696-4704
   Abstract »    Full Text »    PDF »
Nonmuscle Myosin IIA and IIB Have Distinct Functions in the Exocytosis-dependent Process of Cell Membrane Repair.
T. Togo and R. A. Steinhardt (2004)
Mol. Biol. Cell 15, 688-695
   Abstract »    Full Text »    PDF »
Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase.
J.-C. Kuo, J.-R. Lin, J. M. Staddon, H. Hosoya, and R.-H. Chen (2003)
J. Cell Sci. 116, 4777-4790
   Abstract »    Full Text »    PDF »
Retraction in Amoeboid Cell Motility Powered by Cytoskeletal Dynamics.
L. Miao, O. Vanderlinde, M. Stewart, and T. M. Roberts (2003)
Science 302, 1405-1407
   Abstract »    Full Text »    PDF »
Functional Divergence of Human Cytoplasmic Myosin II: KINETIC CHARACTERIZATION OF THE NON-MUSCLE IIA ISOFORM.
M. Kovacs, F. Wang, A. Hu, Y. Zhang, and J. R. Sellers (2003)
J. Biol. Chem. 278, 38132-38140
   Abstract »    Full Text »    PDF »
RacB Regulates Cytoskeletal Function in Dictyostelium spp..
E. Lee, D. J. Seastone, E. Harris, J. A. Cardelli, and D. A. Knecht (2003)
Eukaryot. Cell 2, 474-485
   Abstract »    Full Text »    PDF »
Dictyostelium and Acanthamoeba myosin II assembly domains go to the cleavage furrow of Dictyostelium myosin II-null cells.
S. Shu, X. Liu, and E. D. Korn (2003)
PNAS 100, 6499-6504
   Abstract »    Full Text »    PDF »
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor.
A. F. Straight, A. Cheung, J. Limouze, I. Chen, N. J. Westwood, J. R. Sellers, and T. J. Mitchison (2003)
Science 299, 1743-1747
   Abstract »    Full Text »    PDF »
Cell polarity and locomotion, as well as endocytosis, depend on NSF.
C. R. L. Thompson and M. S. Bretscher (2003)
Development 129, 4185-4192
   Abstract »    Full Text »    PDF »
ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum.
C. Kitayama and T. Q. P. Uyeda (2003)
J. Cell Sci. 116, 711-723
   Abstract »    Full Text »    PDF »
Direct Evidence for a Critical Role of Myosin II in Budding Yeast Cytokinesis and the Evolvability of New Cytokinetic Mechanisms in the Absence of Myosin II.
N. Tolliday, M. Pitcher, and R. Li (2003)
Mol. Biol. Cell 14, 798-809
   Abstract »    Full Text »    PDF »
Myosin II contributes to the posterior contraction and the anterior extension during the retraction phase in migrating Dictyostelium cells.
K. S. K. Uchida, T. Kitanishi-Yumura, and S. Yumura (2003)
J. Cell Sci. 116, 51-60
   Abstract »    Full Text »    PDF »
Calcium regulation of actin crosslinking is important for function of the actin cytoskeleton in Dictyostelium.
R. Furukawa, A. Maselli, S. A. M. Thomson, R. W. L. Lim, J. V. Stokes, and M. Fechheimer (2003)
J. Cell Sci. 116, 187-196
   Abstract »    Full Text »    PDF »
Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation.
M. M. Hossain, D.-Y. Hwang, Q.-Q. Huang, Y. Sasaki, and J.-P. Jin (2003)
Am J Physiol Cell Physiol 284, C156-C167
   Abstract »    Full Text »    PDF »
The Identification of Pats1, a Novel Gene Locus Required for Cytokinesis in Dictyostelium discoideum.
J. C. Abysalh, L. L. Kuchnicki, and D. A. Larochelle (2003)
Mol. Biol. Cell 14, 14-25
   Abstract »    Full Text »    PDF »
Novel Myosin Heavy Chain Kinase Involved in Disassembly of Myosin II Filaments and Efficient Cleavage in Mitotic Dictyostelium Cells.
A. Nagasaki, G. Itoh, S. Yumura, and T. Q.P. Uyeda (2002)
Mol. Biol. Cell 13, 4333-4342
   Abstract »    Full Text »    PDF »
Tail chimeras of Dictyostelium myosin II support cytokinesis and other myosin II activities but not full development.
S. Shu, X. Liu, C. A. Parent, T. Q. P. Uyeda, and E. D. Korn (2002)
J. Cell Sci. 115, 4237-4249
   Abstract »    Full Text »    PDF »
The UCS family of myosin chaperones.
A. H. Hutagalung, M. L. Landsverk, M. G. Price, and H. F. Epstein (2002)
J. Cell Sci. 115, 3983-3990
   Abstract »    Full Text »    PDF »
Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint.
D. P. Mulvihill and J. S. Hyams (2002)
J. Cell Sci. 115, 3575-3586
   Abstract »    Full Text »    PDF »
Cytokinesis in Eukaryotes.
D. A. Guertin, S. Trautmann, and D. McCollum (2002)
Microbiol. Mol. Biol. Rev. 66, 155-178
   Abstract »    Full Text »    PDF »
Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostelium.
A. Nagasaki, E. L. de Hostos, and T. Q. P. Uyeda (2002)
J. Cell Sci. 115, 2241-2251
   Abstract »    Full Text »    PDF »
CHEMOTACTIC SIGNALING PATHWAYS IN NEUTROPHILS: FROM RECEPTOR TO ACTIN ASSEMBLY.
G. Cicchetti, P. G. Allen, and M. Glogauer (2002)
Critical Reviews in Oral Biology & Medicine 13, 220-228
   Abstract »    Full Text »    PDF »
Phosphorylation of the myosin regulatory light chain plays a role in motility and polarity during Dictyostelium chemotaxis.
H. Zhang, D. Wessels, P. Fey, K. Daniels, R. L. Chisholm, and D. R. Soll (2002)
J. Cell Sci. 115, 1733-1747
   Abstract »    Full Text »    PDF »
Dynacortin Is a Novel Actin Bundling Protein That Localizes to Dynamic Actin Structures.
D. N. Robinson, S. S. Ocon, R. S. Rock, and J. A. Spudich (2002)
J. Biol. Chem. 277, 9088-9095
   Abstract »    Full Text »    PDF »
PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.
H. Kuwayama, S. Obara, T. Morio, M. Katoh, H. Urushihara, and Y. Tanaka (2002)
Nucleic Acids Res. 30, e2
   Abstract »    Full Text »    PDF »
Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.
S. Yumura (2001)
J. Cell Biol. 154, 137-146
   Abstract »    Full Text »    PDF »
Ca2+-RhoA signaling pathway required for polyamine-dependent intestinal epithelial cell migration.
J. N. Rao, L. Li, V. A. Golovina, O. Platoshyn, E. D. Strauch, J. X.-J. Yuan, and J.-Y. Wang (2001)
Am J Physiol Cell Physiol 280, C993-C1007
   Abstract »    Full Text »    PDF »
Tortoise, a Novel Mitochondrial Protein, Is Required for Directional Responses of Dictyostelium in Chemotactic Gradients.
S. van Es, D. Wessels, D. R. Soll, J. Borleis, and P. N. Devreotes (2001)
J. Cell Biol. 152, 621-632
   Abstract »    Full Text »    PDF »
Myosin II-dependent cylindrical protrusions induced by quinine in Dictyostelium: antagonizing effects of actin polymerization at the leading edge.
K. Yoshida and K. Inouye (2001)
J. Cell Sci. 114, 2155-2165
   Abstract »    Full Text »    PDF »
Reorganization of actin cytoskeleton at the growing end of the cleavage furrow of Xenopus egg during cytokinesis.
T Noguchi and I Mabuchi (2001)
J. Cell Sci. 114, 401-412
   Abstract »    PDF »
Conditional Expression of a Truncated Fragment of Nonmuscle Myosin II-A Alters Cell Shape but Not Cytokinesis in HeLa Cells.
Q. Wei and R. S. Adelstein (2000)
Mol. Biol. Cell 11, 3617-3627
   Abstract »    Full Text »
Dynacortin, a Genetic Link between Equatorial Contractility and Global Shape Control Discovered by Library Complementation of a Dictyostelium discoideum Cytokinesis Mutant.
D. N. Robinson and J. A. Spudich (2000)
J. Cell Biol. 150, 823-838
   Abstract »    Full Text »    PDF »
The Internal Phosphodiesterase RegA Is Essential for the Suppression of Lateral Pseudopods during Dictyostelium Chemotaxis.
D. J. Wessels, H. Zhang, J. Reynolds, K. Daniels, P. Heid, S. Lu, A. Kuspa, G. Shaulsky, W. F. Loomis, and D. R. Soll (2000)
Mol. Biol. Cell 11, 2803-2820
   Abstract »    Full Text »
LSP1 modulates the locomotion of monocyte-differentiated U937 cells.
Y. Li, Q. Zhang, R. Aaron, L. Hilliard, and T. H. Howard (2000)
Blood 96, 1100-1105
   Abstract »    Full Text »    PDF »
Genetic Analysis Demonstrates a Direct Link Between Rho Signaling and Nonmuscle Myosin Function During Drosophila Morphogenesis.
S. R. Halsell, B. I. Chu, and D. P. Kiehart (2000)
Genetics 155, 1253-1265
   Abstract »    Full Text »
Intracellular Growth of Legionella pneumophila in Dictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions.
J. M. Solomon, A. Rupper, J. A. Cardelli, and R. R. Isberg (2000)
Infect. Immun. 68, 2939-2947
   Abstract »    Full Text »    PDF »
The Dictyostelium LIM Domain-containing Protein LIM2 Is Essential for Proper Chemotaxis and Morphogenesis.
S. Chien, C. Y. Chung, S. Sukumaran, N. Osborne, S. Lee, C. Ellsworth, J. G. McNally, and R. A. Firtel (2000)
Mol. Biol. Cell 11, 1275-1291
   Abstract »    Full Text »
Three-dimensional in vivo analysis of Dictyostelium mounds reveals directional sorting of prestalk cells and defines a role for the myosin II regulatory light chain in prestalk cell sorting and tip protrusion.
P. Clow, T Chen, R. Chisholm, and J. McNally (2000)
Development 127, 2715-2728
   Abstract »    PDF »
Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe.
F Motegi, K Nakano, and I Mabuchi (2000)
J. Cell Sci. 113, 1813-1825
   Abstract »    PDF »
The actin cytoskeleton of Dictyostelium: a story told by mutants.
A. Noegel and M Schleicher (2000)
J. Cell Sci. 113, 759-766
   Abstract »    PDF »
The Dictyostelium RasS protein is required for macropinocytosis, phagocytosis and the control of cell movement.
J. Chubb, A Wilkins, G. Thomas, and R. Insall (2000)
J. Cell Sci. 113, 709-719
   Abstract »    PDF »
Deletion of the Myopathy Loop of Dictyostelium Myosin II and Its Impact on Motor Functions.
N. Sasaki, H. Asukagawa, R. Yasuda, T. Hiratsuka, and K. Sutoh (1999)
J. Biol. Chem. 274, 37840-37844
   Abstract »    Full Text »    PDF »
Differentiated intestinal epithelial cells exhibit increased migration through polyamines and myosin II.
J. N. Rao, J. Li, L. Li, B. L. Bass, and J.-Y. Wang (1999)
Am J Physiol Gastrointest Liver Physiol 277, G1149-G1158
   Abstract »    Full Text »    PDF »
LvsA, a Protein Related to the Mouse Beige Protein, Is Required for Cytokinesis in Dictyostelium.
E. Kwak, N. Gerald, D. A. Larochelle, K. K. Vithalani, M. L. Niswonger, M. Maready, and A. De Lozanne (1999)
Mol. Biol. Cell 10, 4429-4439
   Abstract »    Full Text »
A Structural Model for Phosphorylation Control of Dictyostelium Myosin II Thick Filament Assembly.
W. Liang, H. M. Warrick, and J. A. Spudich (1999)
J. Cell Biol. 147, 1039-1048
   Abstract »    Full Text »    PDF »
Paka, a Putative Pak Family Member, Is Required for Cytokinesis and the Regulation of the Cytoskeleton in Dictyostelium discoideum Cells during Chemotaxis.
C. Y. Chung and R. A. Firtel (1999)
J. Cell Biol. 147, 559-576
   Abstract »    Full Text »    PDF »
Engineering of the Myosin-Ibeta Nucleotide-binding Pocket to Create Selective Sensitivity to N6-modified ADP Analogs.
P. G. Gillespie, S. K. H. Gillespie, J. A. Mercer, K. Shah, and K. M. Shokat (1999)
J. Biol. Chem. 274, 31373-31381
   Abstract »    Full Text »    PDF »
Disturbed Communication between Actin- and Nucleotide-binding Sites in a Myosin II with Truncated 50/20-kDa Junction.
M. L. W. Knetsch, T. Q. P. Uyeda, and D. J. Manstein (1999)
J. Biol. Chem. 274, 20133-20138
   Abstract »    Full Text »    PDF »
Phosphorylation of the Myosin-II Light Chain Does Not Regulate the Timing of Cytokinesis in Fission Yeast.
D. McCollum, A. Feoktistova, and K. L. Gould (1999)
J. Biol. Chem. 274, 17691-17695
   Abstract »    Full Text »    PDF »
Glioma Migration Can Be Blocked by Nontoxic Inhibitors of Myosin II.
G. Y. Gillespie, L. Soroceanu, T. J. Manning Jr., C. L. Gladson, and S. S. Rosenfeld (1999)
Cancer Res. 59, 2076-2082
   Abstract »    Full Text »    PDF »
In Vivo Observations of Myosin II Dynamics Support a Role in Rear Retraction.
P. A. Clow and J. G. McNally (1999)
Mol. Biol. Cell 10, 1309-1323
   Abstract »    Full Text »
Identification of a Suppressor of the Dictyostelium Profilin-minus Phenotype as a CD36/LIMP-II Homologue.
I. Karakesisoglou, K.-P. Janssen, L. Eichinger, A. A. Noegel, and M. Schleicher (1999)
J. Cell Biol. 145, 167-181
   Abstract »    Full Text »    PDF »
The Multiple Roles of Cyk1p in the Assembly and Function of the Actomyosin Ring in Budding Yeast.
K. B. Shannon and R. Li (1999)
Mol. Biol. Cell 10, 283-296
   Abstract »    Full Text »
Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium.
S Shu, R. Lee, J. LeBlanc-Straceski, and T. Uyeda (1999)
J. Cell Sci. 112, 2195-2201
   Abstract »    PDF »
Chlamydomonas: The Cell and Its Genomes.
P. A. Lefebvre and C. D. Silflow (1999)
Genetics 151, 9-14
   Full Text »
Disruption of a Dynamin Homologue Affects Endocytosis, Organelle Morphology, and Cytokinesis in Dictyostelium discoideum.
D. C. Wienke, M. L.W. Knetsch, E. M. Neuhaus, M. C. Reedy, and D. J. Manstein (1999)
Mol. Biol. Cell 10, 225-243
   Abstract »    Full Text »
Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation.
X. Liu, K. Ito, S. Morimoto, A. Hikkoshi-Iwane, T. Yanagida, and T. Q. P. Uyeda (1998)
PNAS 95, 14124-14129
   Abstract »    Full Text »    PDF »
Myosin II localization during cytokinesis occurs by a mechanism that does not require its motor domain.
J.-H. Zang and J. A. Spudich (1998)
PNAS 95, 13652-13657
   Abstract »    Full Text »    PDF »
Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylation.
S. Cai, L. Pestic-Dragovich, M. E. O'Donnell, N. Wang, D. Ingber, E. Elson, and P. De Lanerolle (1998)
Am J Physiol Cell Physiol 275, C1349-C1356
   Abstract »    Full Text »    PDF »
Identification of Darlin, a Dictyostelium Protein with Armadillo-like Repeats that Binds to Small GTPases and Is Important for the Proper Aggregation of Developing Cells.
K. K. Vithalani, C. A. Parent, E. M. Thorn, M. Penn, D. A. Larochelle, P. N. Devreotes, and A. De Lozanne (1998)
Mol. Biol. Cell 9, 3095-3106
   Abstract »    Full Text »
A conventional myosin motor drives neurite outgrowth.
S. R. Wylie, P.-j. Wu, H. Patel, and P. D. Chantler (1998)
PNAS 95, 12967-12972
   Abstract »    Full Text »    PDF »
MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway.
L. A. Silveira, J. L. Smith, J. L. Tan, and J. A. Spudich (1998)
PNAS 95, 13000-13005
   Abstract »    Full Text »    PDF »
Involvement of an Actomyosin Contractile Ring in Saccharomyces cerevisiae Cytokinesis.
E. Bi, P. Maddox, D. J. Lew, E.D. Salmon, J. N. McMillan, E. Yeh, and J. R. Pringle (1998)
J. Cell Biol. 142, 1301-1312
   Abstract »    Full Text »    PDF »
SCAR, a WASP-related Protein, Isolated as a Suppressor of Receptor Defects in Late Dictyostelium Development.
J. E. Bear, J. F. Rawls, and C. L. Saxe III (1998)
J. Cell Biol. 142, 1325-1335
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)