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Science 15 May 1987:
Vol. 236. no. 4803, pp. 840 - 843
DOI: 10.1126/science.3554510
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Articles

Science, Vol 236, Issue 4803, 840-843
Copyright © 1987 by American Association for the Advancement of Science

articles

Ras p21 as a potential mediator of insulin action in Xenopus oocytes

LJ Korn, CW Siebel, F McCormick, and RA Roth

The oncogene protein product (p21) of the ras gene has been implicated in mediating the effects of a variety of growth factors and hormones. Microinjection of monoclonal antibody 6B7, which is directed against a synthetic peptide corresponding to a highly conserved region of p21 (amino acids 29 to 44) required for p21 function, specifically inhibited Xenopus oocyte maturation induced by incubation with insulin. The inhibition was dose-dependent and specific since (i) the same antibody had no effect on progesterone-induced maturation, (ii) immunoprecipitation and Western blotting indicated that the antibody recognized a single protein of molecular weight 21,000 in oocyte extracts, and (iii) inhibition was not observed with identical concentrations of normal immunoglobulin. Thus, p21 appears to be involved in mediating insulin-induced maturation of Xenopus oocytes. Furthermore, the mechanism may involve phosphorylation of p21, as p21 was found to be a substrate of the insulin receptor kinase.


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Binding of the Ras activator son of sevenless to insulin receptor substrate-1 signaling complexes.
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Mos and Ras: Two Oncoproteins That Display M-phase Activity.
I. Daar, R. Zhou, R.-L. Shen, A. Nebreda, M. Oskarsson, E. Santos, P. Pinto da Silva, Y. Masui, and G.F. Vande Woude (1991)
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B. D. Nelkin, A. C. de Bustros, M. Mabry, and S. B. Baylin (1989)
JAMA 261, 3130-3135
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Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
M. Low and A. Saltiel (1988)
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Critical Role of Cellular ras Proteins in Proliferative Signal Transduction.
D.W. Stacey, M.-H. Tsai, C.-L. Yu, and J.K. Smith (1988)
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