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Science 1 May 1987:
Vol. 236. no. 4801, pp. 586 - 589
DOI: 10.1126/science.3107122
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Articles

Science, Vol 236, Issue 4801, 586-589
Copyright © 1987 by American Association for the Advancement of Science

articles

Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis

RV Farese, TS Konda, JS Davis, ML Standaert, RJ Pollet, and DR Cooper

The mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes were examined. When [3H]arachidonate labeling of phospholipids was used as an indicator of phospholipase C activation, transient increases in [3H]diacylglycerol were observed between 0.5 and 10 minutes after the onset of insulin treatment. With [3H]glycerol labeling as an indicator of de novo phospholipid synthesis, [3H]diacylglycerol was increased maximally at 1 minute and remained elevated for 20 minutes. [3H]Glycerol-labeled diacylglycerol was largely derived directly from phosphatidic acid. Insulin increased de novo phosphatidic acid synthesis within 5 to 10 seconds; within 1 minute, this synthesis was 60 times greater than that of controls. Thus, the initial increase in diacylglycerol is due to both increased hydrolysis of phospholipids and a burst of de novo phosphatidic acid synthesis. After 5 to 10 minutes, de novo phosphatidic acid synthesis continues as a major source of diacylglycerol. Both phospholipid effects of insulin seem important for generating diacylglycerol and other phospholipid-derived intracellular signaling substances.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Hepatic Expression of ErbB3 Is Repressed by Insulin in a Pathway Sensitive to PI-3 Kinase Inhibitors.
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Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
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