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Science 1 May 1987:
Vol. 236. no. 4801, pp. 573 - 576
DOI: 10.1126/science.3033823
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Articles

Science, Vol 236, Issue 4801, 573-576
Copyright © 1987 by American Association for the Advancement of Science

articles

Clustering of genes dispensable for growth in culture in the S component of the HSV-1 genome

R Longnecker and B Roizman

The herpes simplex virus 1 genome consists of one long and one short stretch of unique sequences flanked by inverted repeat sequences. The nucleotide sequence and RNA map predict 12 open reading frames designated as US1 through US12 within the short stretch of unique sequences. This paper reports the construction of virus mutants from which US2, US3, or US4 had been deleted that are capable of growth in cell culture. One of the three deleted genes, US4, specifies the viral envelope glycoprotein G. Mutants with deletions in US1, US8, US9, US10, US11, and US12 have been previously reported. The nine genes deleted from this region form two clusters, US1 through US4 and US8 through US12, and encode at least two and possibly more structural proteins. The presence of so many genes dispensable for growth in cell culture suggests several hypotheses regarding their function and evolution.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Translocation and Colocalization of ICP4 and ICP0 in Cells Infected with Herpes Simplex Virus 1 Mutants Lacking Glycoprotein E, Glycoprotein I, or the Virion Host Shutoff Product of the UL41 Gene.
M. Kalamvoki, J. Qu, and B. Roizman (2008)
J. Virol. 82, 1701-1713
   Abstract »    Full Text »    PDF »
Horizontal Transmission of Marek's Disease Virus Requires US2, the UL13 Protein Kinase, and gC.
K. W. Jarosinski, N. G. Margulis, J. P. Kamil, S. J. Spatz, V. K. Nair, and N. Osterrieder (2007)
J. Virol. 81, 10575-10587
   Abstract »    Full Text »    PDF »
Bcl-2 Blocks Accretion or Depletion of Stored Calcium but Has No Effect on the Redistribution of IP3 Receptor I Mediated by Glycoprotein E of Herpes Simplex Virus 1.
M. Kalamvoki and B. Roizman (2007)
J. Virol. 81, 6316-6325
   Abstract »    Full Text »    PDF »
Mapping of Key Functions of the Herpes Simplex Virus 1 US3 Protein Kinase: the US3 Protein Can Form Functional Heteromultimeric Structures Derived from Overlapping Truncated Polypeptides.
A. P. W. Poon and B. Roizman (2007)
J. Virol. 81, 1980-1989
   Abstract »    Full Text »    PDF »
Glycoprotein G is a virulence factor in infectious laryngotracheitis virus..
J. M. Devlin, G. F. Browning, C. A. Hartley, N. C. Kirkpatrick, A. Mahmoudian, A. H. Noormohammadi, and J. R. Gilkerson (2006)
J. Gen. Virol. 87, 2839-2847
   Abstract »    Full Text »    PDF »
Localization of ERK/MAP Kinase Is Regulated by the Alphaherpesvirus Tegument Protein Us2.
M. G. Lyman, J. A. Randall, C. M. Calton, and B. W. Banfield (2006)
J. Virol. 80, 7159-7168
   Abstract »    Full Text »    PDF »
Construction and properties of a herpes simplex virus 1 designed to enter cells solely via the IL-13{alpha}2 receptor.
G. Zhou and B. Roizman (2006)
PNAS 103, 5508-5513
   Abstract »    Full Text »    PDF »
The Extracellular Domain of Herpes Simplex Virus gE Is Indispensable for Efficient Cell-to-Cell Spread: Evidence for gE/gI Receptors.
K. Polcicova, K. Goldsmith, B. L. Rainish, T. W. Wisner, and D. C. Johnson (2005)
J. Virol. 79, 11990-12001
   Abstract »    Full Text »    PDF »
Expression and Localization of the Epstein-Barr Virus-Encoded Protein Kinase.
E. Gershburg, M. Marschall, K. Hong, and J. S. Pagano (2004)
J. Virol. 78, 12140-12146
   Abstract »    Full Text »    PDF »
Herpes Simplex Virus Type 1 Primary Envelopment: UL34 Protein Modification and the US3-UL34 Catalytic Relationship.
B. J. Ryckman and R. J. Roller (2004)
J. Virol. 78, 399-412
   Abstract »    Full Text »    PDF »
The Pseudorabies Virus Us2 Protein, a Virion Tegument Component, Is Prenylated in Infected Cells.
A. C. Clase, M. G. Lyman, T. del Rio, J. A. Randall, C. M. Calton, L. W. Enquist, and B. W. Banfield (2003)
J. Virol. 77, 12285-12298
   Abstract »    Full Text »    PDF »
Genome of Bovine Herpesvirus 5.
G. Delhon, M. P. Moraes, Z. Lu, C. L. Afonso, E. F. Flores, R. Weiblen, G. F. Kutish, and D. L. Rock (2003)
J. Virol. 77, 10339-10347
   Abstract »    Full Text »    PDF »
Herpes Simplex Virus Glycoproteins gD and gE/gI Serve Essential but Redundant Functions during Acquisition of the Virion Envelope in the Cytoplasm.
A. Farnsworth, K. Goldsmith, and D. C. Johnson (2003)
J. Virol. 77, 8481-8494
   Abstract »    Full Text »    PDF »
The Herpes Simplex Virus Type 1 US11 Protein Binds the Coterminal UL12, UL13, and UL14 RNAs and Regulates UL13 Expression In Vivo.
H. L. Attrill, S. A. Cumming, J. B. Clements, and S. V. Graham (2002)
J. Virol. 76, 8090-8100
   Abstract »    Full Text »    PDF »
Herpes Simplex Virus Tegument Protein US11 Interacts with Conventional Kinesin Heavy Chain.
R. J. Diefenbach, M. Miranda-Saksena, E. Diefenbach, D. J. Holland, R. A. Boadle, P. J. Armati, and A. L. Cunningham (2002)
J. Virol. 76, 3282-3291
   Abstract »    Full Text »    PDF »
Insertions in the gG Gene of Pseudorabies Virus Reduce Expression of the Upstream Us3 Protein and Inhibit Cell-to-Cell Spread of Virus Infection.
G. L. Demmin, A. C. Clase, J. A. Randall, L. W. Enquist, and B. W. Banfield (2001)
J. Virol. 75, 10856-10869
   Abstract »    Full Text »    PDF »
UL31 and UL34 Proteins of Herpes Simplex Virus Type 1 Form a Complex That Accumulates at the Nuclear Rim and Is Required for Envelopment of Nucleocapsids.
A. E. Reynolds, B. J. Ryckman, J. D. Baines, Y. Zhou, L. Liang, and R. J. Roller (2001)
J. Virol. 75, 8803-8817
   Abstract »    Full Text »    PDF »
The C-terminal cytoplasmic tail of herpes simplex virus type 1 gE protein is phosphorylated in vivo and in vitro by cellular enzymes in the absence of other viral proteins.
V. Miriagou, L. Stevanato, R. Manservigi, and P. Mavromara (2000)
J. Gen. Virol. 81, 1027-1031
   Abstract »    Full Text »
The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse.
J. F. Moffat, L. Zerboni, M. H. Sommer, T. C. Heineman, J. I. Cohen, H. Kaneshima, and A. M. Arvin (1998)
PNAS 95, 11969-11974
   Abstract »    Full Text »    PDF »
HveA (Herpesvirus Entry Mediator A), a Coreceptor for Herpes Simplex Virus Entry, also Participates in Virus-Induced Cell Fusion.
T. Terry-Allison, R. I. Montgomery, J. C. Whitbeck, R. Xu, G. H. Cohen, R. J. Eisenberg, and P. G. Spear (1998)
J. Virol. 72, 5802-5810
   Abstract »    Full Text »    PDF »
UL27.5 Is a Novel gamma 2 Gene Antisense to the Herpes Simplex Virus 1 Gene Encoding Glycoprotein B.
Y. E. Chang, L. Menotti, F. Filatov, G. Campadelli-Fiume, and B. Roizman (1998)
J. Virol. 72, 6056-6064
   Abstract »    Full Text »    PDF »


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