Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 April 1987:
Vol. 236. no. 4797, pp. 77 - 81
DOI: 10.1126/science.3105056
Prev | Table of Contents | Next

Articles

Science, Vol 236, Issue 4797, 77-81
Copyright © 1987 by American Association for the Advancement of Science

articles

Human lymphocytes making rheumatoid factor and antibody to ssDNA belong to Leu-1+ B-cell subset

P Casali, SE Burastero, M Nakamura, G Inghirami, and AL Notkins

B lymphocytes bearing the Leu-1 cell-surface antigen (Leu-1+), the human equivalent of mouse Ly-1+ B lymphocytes, have been detected in human peripheral blood, but there is little information on their frequency and properties. Analysis by fluorescence-activated cell sorter and double immunofluorescence showed that Leu-1+ B cells are consistently present in the peripheral blood and spleens of healthy subjects and constitute 17.0 +/- 5.0% (mean value +/- standard deviation) and 17.3 +/- 3.9%, respectively, of total B cells. When purified Leu-1+ and Leu-1- B lymphocytes were transformed into immunoglobulin-secreting cells by infection with Epstein-Barr virus and the culture fluids were tested for reactivity with self-antigens, at least two important autoantibodies, antibody to the Fc fragment of human immunoglobulin G (rheumatoid factor) and antibody to single-stranded DNA, were found to be made exclusively by Leu-1+ B cells. It is concluded that the Leu-1+ lymphocytes represent a major subset of the normal human B cell repertoire and include the B cells capable of making autoantibodies similar to those found in systemic lupus erythematosus and rheumatoid arthritis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and Characterization of a Human CD5+ Pre-Naive B Cell Population.
J. Lee, S. Kuchen, R. Fischer, S. Chang, and P. E. Lipsky (2009)
J. Immunol. 182, 4116-4126
   Abstract »    Full Text »    PDF »
A Subset of Host B Lymphocytes Controls Melanoma Metastasis through a Melanoma Cell Adhesion Molecule/MUC18-Dependent Interaction: Evidence from Mice and Humans.
F. I. Staquicini, A. Tandle, S. K. Libutti, J. Sun, M. Zigler, M. Bar-Eli, F. Aliperti, E. C. Perez, J. E. Gershenwald, M. Mariano, et al. (2008)
Cancer Res. 68, 8419-8428
   Abstract »    Full Text »    PDF »
The endogenous danger signal, crystalline uric acid, signals for enhanced antibody immunity.
M. D. Behrens, W. M. Wagner, C. J. Krco, C. L. Erskine, K. R. Kalli, J. Krempski, E. A. Gad, M. L. Disis, and K. L. Knutson (2008)
Blood 111, 1472-1479
   Abstract »    Full Text »    PDF »
Human endometriosis is associated with plasma cells and overexpression of B lymphocyte stimulator.
A. Hever, R. B. Roth, P. Hevezi, M. E. Marin, J. A. Acosta, H. Acosta, J. Rojas, R. Herrera, D. Grigoriadis, E. White, et al. (2007)
PNAS 104, 12451-12456
   Abstract »    Full Text »    PDF »
Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
M. C. van Zelm, T. Szczepanski, M. van der Burg, and J. J.M. van Dongen (2007)
J. Exp. Med. 204, 645-655
   Abstract »    Full Text »    PDF »
B-1 B cell development..
R. R. Hardy (2006)
J. Immunol. 177, 2749-2754
   Abstract »    Full Text »    PDF »
Thematic review series: The Immune System and Atherogenesis. The role of natural antibodies in atherogenesis.
C. J. Binder, P. X. Shaw, M.-K. Chang, A. Boullier, K. Hartvigsen, S. Horkko, Y. I. Miller, D. A. Woelkers, M. Corr, and J. L. Witztum (2005)
J. Lipid Res. 46, 1353-1363
   Abstract »    Full Text »    PDF »
Predictive value of anti-cell and anti-human immunodeficiency virus (HIV) humoral responses in HIV-1-exposed seronegative cohorts of European and Asian origin.
L. Lopalco, C. Barassi, C. Paolucci, D. Breda, D. Brunelli, M. Nguyen, J. Nouhin, T. T. Luong, L. X. Truong, M. Clerici, et al. (2005)
J. Gen. Virol. 86, 339-348
   Abstract »    Full Text »    PDF »
Molecular Recognition Patterns of Serum Anti-DNA Topoisomerase I Antibody in Systemic Sclerosis.
P. Q. Hu, N. Fertig, T. A. Medsger Jr, and T. M. Wright (2004)
J. Immunol. 173, 2834-2841
   Abstract »    Full Text »    PDF »
Functional and Morphological Development of Lymphoid Tissues and Immune Regulatory and Effector Function in Rhesus Monkeys: Cytokine-Secreting Cells, Immunoglobulin-Secreting Cells, and CD5+ B-1 Cells Appear Early in Fetal Development.
N. Makori, A. F. Tarantal, F. X. Lu, T. Rourke, M. L. Marthas, M. B. McChesney, A. G. Hendrickx, and C. J. Miller (2003)
Clin. Vaccine Immunol. 10, 140-153
   Abstract »    Full Text »    PDF »
Ongoing In Vivo Immunoglobulin Class Switch DNA Recombination in Chronic Lymphocytic Leukemia B Cells.
A. Cerutti, H. Zan, E. C. Kim, S. Shah, E. J. Schattner, A. Schaffer, and P. Casali (2002)
J. Immunol. 169, 6594-6603
   Abstract »    Full Text »    PDF »
Peritoneal CD5+ B-1 Cells Have Signaling Properties Similar to Tolerant B Cells.
S.-C. Wong, W.-K. Chew, J. E.-L. Tan, A. J. Melendez, F. Francis, and K.-P. Lam (2002)
J. Biol. Chem. 277, 30707-30715
   Abstract »    Full Text »    PDF »
Differentially Regulated Expression and Function of CD22 in Activated B-1 and B-2 Lymphocytes.
F. Lajaunias, L. Nitschke, T. Moll, E. Martinez-Soria, I. Semac, Y. Chicheportiche, R. M. E. Parkhouse, and S. Izui (2002)
J. Immunol. 168, 6078-6083
   Abstract »    Full Text »    PDF »
Lupus-Specific Antiribonucleoprotein B Cell Tolerance in Nonautoimmune Mice Is Maintained by Differentiation to B-1 and Governed by B Cell Receptor Signaling Thresholds.
Y. Qian, C. Santiago, M. Borrero, T. F. Tedder, and S. H. Clarke (2001)
J. Immunol. 166, 2412-2419
   Abstract »    Full Text »    PDF »
Analysis of anti-neutrophil cytoplasmic antibodies (ANCA): frequency and specificity in a sample of 191 homozygous (PiZZ) alpha1-antitrypsin-deficient subjects.
M. A. P. Audrain, R. Sesboue, T. A. R. Baranger, J. Elliott, A. Testa, J.-P. Martin, C. M. Lockwood, and V. L. M. Esnault (2001)
Nephrol. Dial. Transplant. 16, 39-44
   Abstract »    Full Text »    PDF »
Migration and Differentiation of Autoreactive B-1 Cells Induced by Activated {gamma}/{delta} T Cells in Antierythrocyte Immunoglobulin Transgenic Mice.
N. Watanabe, K. Ikuta, S. Fagarasan, S. Yazumi, T. Chiba, and T. Honjo (2000)
J. Exp. Med. 192, 1577-1586
   Abstract »    Full Text »    PDF »
Identification of a Precursor to Phosphatidyl Choline-Specific B-1 Cells Suggesting That B-1 Cells Differentiate from Splenic Conventional B Cells In Vivo: Cyclosporin A Blocks Differentiation to B-1.
L. W. Arnold, S. K. McCray, C. Tatu, and S. H. Clarke (2000)
J. Immunol. 164, 2924-2930
   Abstract »    Full Text »    PDF »
Selection at Multiple Checkpoints Focuses VH12 B Cell Differentiation toward a Single B-1 Cell Specificity.
C. Tatu, J. Ye, L. W. Arnold, and S. H. Clarke (1999)
J. Exp. Med. 190, 903-914
   Abstract »    Full Text »    PDF »
Human Hematopoietic Stem/Progenitor Cells Generate CD5+ B Lymphoid Cells in NOD/SCID Mice.
E. M. Novelli, M. Ramírez, W. Leung, and C. I. Civin (1999)
Stem Cells 17, 242-252
   Abstract »    Full Text »
CD5 Negatively Regulates the T-Cell Antigen Receptor Signal Transduction Pathway: Involvement of SH2-Containing Phosphotyrosine Phosphatase SHP-1.
J. J. Perez-Villar, G. S. Whitney, M. A. Bowen, D. H. Hewgill, A. A. Aruffo, and S. B. Kanner (1999)
Mol. Cell. Biol. 19, 2903-2912
   Abstract »    Full Text »    PDF »
Innate and acquired humoral immunities to influenza virus are mediated by distinct arms of the immune system.
N. Baumgarth, O. C. Herman, G. C. Jager, L. Brown, L. A. Herzenberg, and L. A. Herzenberg (1999)
PNAS 96, 2250-2255
   Abstract »    Full Text »    PDF »
Do Antibodies Stimulate Myelin Repair in Multiple Sclerosis?.
D. Mcgavern, K. Asakura, and M. Rodriquez (1999)
Neuroscientist 5, 19-28
   Abstract »    PDF »
Susceptibility Alleles for Aberrant B-1 Cell Proliferation Involved in Spontaneously Occurring B-Cell Chronic Lymphocytic Leukemia in a Model of New Zealand White Mice.
Y. Hamano, S. Hirose, A. Ida, M. Abe, D. Zhang, S. Kodera, Y. Jiang, J. Shirai, Y. Miura, H. Nishimura, et al. (1998)
Blood 92, 3772-3779
   Abstract »    Full Text »    PDF »
Genetic and Immunological Properties of Phage-Displayed Human Anti-Rh(D) Antibodies: Implications for Rh(D) Epitope Topology.
T. Y. Chang and D. L. Siegel (1998)
Blood 91, 3066-3078
   Abstract »    Full Text »    PDF »
Lack of Intraclonal Diversification in Ig Heavy and Light Chain V Region Genes Expressed by CD5+IgM+ Chronic Lymphocytic Leukemia B Cells: A Multiple Time Point Analysis.
E. W. Schettino, A. Cerutti, N. Chiorazzi, and P. Casali (1998)
J. Immunol. 160, 820-830
   Abstract »    Full Text »    PDF »
When Do Microbes Stimulate Rheumatoid Factor?.
D. N. Posnett and J. Edinger (1997)
J. Exp. Med. 185, 1721-1723
   Full Text »    PDF »
A Chronic Illness Characterized by Fatigue, Neurologic and Immunologic Disorders, and Active Human Herpesvirus Type 6 Infection.
D. Buchwald, P. R. Cheney, D. L. Peterson, B. Henry, S. B. Wormsley, A. Geiger, D. V. Ablashi, S. Z. Salahuddin, C. Saxinger, R. Biddle, et al. (1992)
Ann Intern Med 116, 103-113
   Abstract »    PDF »
Chronic Lymphocytic Leukemia: New Insights into Biology and Therapy.
K. A. Foon, K. R. Rai, and R. P. Gale (1990)
Ann Intern Med 113, 525-539
   Abstract »    PDF »
Increased Numbers of CD5 B Lymphocytes in Schizophrenic Patients.
C. G. McAllister, M. H. Rapaport, D. Pickar, T. A. Podruchny, G. Christison, L. D. Alphs, and S. M. Paul (1989)
Arch Gen Psychiatry 46, 890-894
   Abstract »    PDF »
Effects of Short-term Administration of Antipsychotic Drugs on Lymphocyte Subsets in Schizophrenic Patients.
C. G. McAllister, M. H. Rapaport, D. Pickar, and S. M. Paul (1989)
Arch Gen Psychiatry 46, 956-957
   Abstract »    PDF »
Elevated Levels of Soluble Interleukin 2 Receptors in Schizophrenia.
M. H. Rapaport, C. G. McAllister, D. Pickar, D. L. Nelson, and S. M. Paul (1989)
Arch Gen Psychiatry 46, 291-292
   Abstract »    PDF »
The Biology of the Immune Response.
H. N. Claman (1987)
JAMA 258, 2834-2840
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)