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Science 27 March 1987:
Vol. 235. no. 4796, pp. 1622 - 1628
DOI: 10.1126/science.3029873
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Articles

Science, Vol 235, Issue 4796, 1622-1628
Copyright © 1987 by American Association for the Advancement of Science

articles

Oncogenesis of the lens in transgenic mice

KA Mahon, AB Chepelinsky, JS Khillan, PA Overbeek, J Piatigorsky, and H Westphal

Neoplastic tumors of the ocular lens of vertebrates do not naturally occur. Transgenic mice carrying a hybrid gene comprising the murine alpha A-crystallin promoter (-366 to +46) fused to the coding sequence of the SV40 T antigens developed lens tumors, which obliterated the eye cavity and even invaded neighboring tissue, thus establishing that the lens is not refractive to oncogenesis. Large-T antigen was detected early in lens development; it elicited morphological changes and specifically interfered with differentiation of lens fiber cells. Both alpha- and beta-crystallins persisted in many of the lens tumor cells, while gamma-crystallin was selectively reduced. Accessibility, characteristic morphology, and defined protein markers make this transparent epithelial eye tissue a potentially useful system for testing tumorigenicity of oncogenes and for studying malignant transformation from its inception until death of the animal.


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Chick {delta}1-Crystallin Enhancer Influences Mouse {alpha}A-Crystallin Promoter Activity in Transgenic Mice.
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Manipulating the germline: its impact on the study of carcinogenesis.
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Transport of fluid by lens epithelium.
J. Fischbarg, F. P. J. Diecke, K. Kuang, B. Yu, F. Kang, P. Iserovich, Y. Li, H. Rosskothen, and J. P. Koniarek (1999)
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Studies in Transgenic Mice Reveal Potential Relationships between Secretin-producing Cells and Other Endocrine Cell Types.
M. J. Lopez, B. H. Upchurch, G. Rindi, and A. B. Leiter (1995)
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Altered cell cycle regulation in the lens of HPV-16 E6 or E7 transgenic mice: implications for tumor suppressor gene function in development..
H Pan and A E Griep (1994)
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Expression of peptide YY in all four islet cell types in the developing mouse pancreas suggests a common peptide YY-producing progenitor.
B. Upchurch, G. Aponte, and A. Leiter (1994)
Development 120, 245-252
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Defective lens fiber differentiation and pancreatic tumorigenesis caused by ectopic expression of the cellular retinoic acid-binding protein I.
A. Perez-Castro, V. Tran, and M. Nguyen-Huu (1993)
Development 119, 363-375
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Transgenic models of tumor development.
J. Adams and S Cory (1991)
Science 254, 1161-1167
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Transgenic mice as probes into complex systems.
D Hanahan (1989)
Science 246, 1265-1275
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Perturbed development of the mouse lens by polyomavirus large T antigen does not lead to tumor formation..
A E Griep, T Kuwabara, E J Lee, and H Westphal (1989)
Genes & Dev. 3, 1075-1085
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Lens-specific expression of recombinant ricin induces developmental defects in the eyes of transgenic mice..
C P Landel, J Zhao, D Bok, and G A Evans (1988)
Genes & Dev. 2, 1168-1178
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Transgenic animals.
R Jaenisch (1988)
Science 240, 1468-1474
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Atrial natriuretic factor-SV40 T antigen transgenes produce tumors and cardiac arrhythmias in mice.
L. Field (1988)
Science 239, 1029-1033
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Defects in lens fiber differentiation are linked to c-mos overexpression in transgenic mice..
J S Khillan, M K Oskarsson, F Propst, T Kuwabara, G F Vande Woude, and H Westphal (1987)
Genes & Dev. 1, 1327-1335
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Pancreatic neoplasia induced by SV40 T-antigen expression in acinar cells of transgenic mice.
D. Ornitz, R. Hammer, A Messing, R. Palmiter, and R. Brinster (1987)
Science 238, 188-193
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