Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 20 March 1987:
Vol. 235. no. 4795, pp. 1498 - 1501
DOI: 10.1126/science.3029871
Prev | Table of Contents | Next

Articles

Science, Vol 235, Issue 4795, 1498-1501
Copyright © 1987 by American Association for the Advancement of Science

articles

The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers

TG Parslow, SD Jones, B Bond, and KR Yamamoto

The thymidine kinase (tk) promoter of herpes simplex virus includes an octanucleotide sequence motif (ATTTGCAT) that is also an essential component of immunoglobulin kappa gene promoters. In the absence of an enhancer, tk promoter derivatives that contain this element support a higher rate of transcription than those that lack it. The action of the kappa enhancer augments that of the octanucleotide in B lymphoid cells; when both elements are present, tk promoter activity is increased by more than an order of magnitude. In contrast, the presence of the octanucleotide in this promoter markedly reduces its response to a nonimmunoglobulin enhancer. These results suggest that the octanucleotide may mediate a selective interaction among promoters and enhancers.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Repression of the IgH enhancer in teratocarcinoma cells associated with a novel octamer factor.
M. Lenardo, L Staudt, P Robbins, A Kuang, R. Mulligan, and D Baltimore (1989)
Science 243, 544-546
   Abstract »    PDF »
Octamer-binding proteins from B or HeLa cells stimulate transcription of the immunoglobulin heavy-chain promoter in vitro..
J H LeBowitz, T Kobayashi, L Staudt, D Baltimore, and P A Sharp (1988)
Genes & Dev. 2, 1227-1237
   Abstract »    PDF »
Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
L. Staudt, R. Clerc, H Singh, J. LeBowitz, P. Sharp, and D Baltimore (1988)
Science 241, 577-580
   Abstract »    PDF »
Distinct factors with Sp1 and NF-A specificities bind to adjacent functional elements of the human U2 snRNA gene enhancer..
M Ares, J S Chung, L Giglio, and A M Weiner (1987)
Genes & Dev. 1, 808-817
   Abstract »    PDF »
Regulation of inducible and tissue-specific gene expression.
T Maniatis, S Goodbourn, and J. Fischer (1987)
Science 236, 1237-1245
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)